Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its (a) accession number, (b) entry name and (c) release date of last modification.
Ans. (a) accession number = P40692 (b) entry name =MLH1_HUMAN (c) release date of last modification : 31, February 1995(Rel.31, Last sequence update) ; 16 October 2001 (Rel. 40, Last annotation update) answer page

Give its number of amino acids, molecular weight and theoretical pI.
Ans. number of amino acids= 756 AA. answer page ; molecular weught = 84600Da ; theoretical pI = 5.51 answer page

Calculate the total number of negatively charged residues and positively charged residues.
Ans. total number of negatively charged residues (Asp+Glu) = 104 ; total number of positively charged residues (Arg+Lys) = 83 answer page

Calculate its hydrophobicity (Kyte & Doolittle scale, window size 11, Relative weight 60%).
Ans. MLH1 hydrophobicity

Performe the trypsin (higher specificity) cleavage of the protein. (a) How many peptides will you get after cleavage? (b) Give the list of peptides with a mass bigger than 1000 dalton.
Ans. (a) 81 (b) list of peptides with a mass bigger than 1000 dalton