LOCUS D88622 6072 bp DNA SYN 02-JUL-1997 DEFINITION Bicistronic retroviral vector. ACCESSION D88622 NID g2243113 KEYWORDS Bicistronic retroviral vector; Mo-MuLV. SOURCE Artificial gene DNA. ORGANISM synthetic construct artificial sequence. REFERENCE 1 (bases 1 to 6072) AUTHORS Akagi,T. TITLE Direct Submission JOURNAL Submitted (28-OCT-1996) to the DDBJ/EMBL/GenBank databases. Tsuyoshi Akagi, National Cancer Center Research Institute, Virology Division; Tsukiji 5-1-1, Chuo-ku, Tokyo 104, Japan (E-mail:takagi@ncc.go.jp, Tel:+81-3-3542-2511, Fax:+81-3-3543-2181) REFERENCE 2 (sites) AUTHORS Akagi,T. TITLE Expression of mutant Tax1 of HTLV-I by bicistronic retroviral vector JOURNAL Unpublished (1997) REFERENCE 3 (sites) AUTHORS Akagi,T., Ono,H., Nyunoya,H. and Shimotohno,K. TITLE Characterization of peripheral blood T-lymphocytes transduced with HTLV-I Tax mutants with different trans-activating phenotypes JOURNAL Oncogene 14 (17), 2071-2078 (1997) MEDLINE 97304524 FEATURES Location/Qualifiers source 1..6072 /organism="synthetic construct" /note="bicistronic Mo-MuLV-based retroviral vector" /db_xref="taxon:32630" LTR 175..763 /note="5' LTR" RBS 1649..2263 /note="IRES sequence from ECMV" misc_feature 2264..3075 /gene="neo" /note="derived from transposon Tn5" /function="kanamycin resistance" gene 2264..3075 /gene="neo" LTR 3118..3711 /note="3' LTR" BASE COUNT 1399 a 1659 c 1563 g 1451 t ORIGIN 1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa 181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa 241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata 301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag 361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg 421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca 481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca 541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc 601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat 661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg 721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga 781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta 901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac 961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc 1621 cctcactcct tctctaggcg ccggaattcg ttaactaact taagctagca acggtttccc 1681 tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata 1741 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt 1801 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct 1861 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc 1921 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga 1981 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc 2041 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt 2101 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg 2161 gcctcggtgc acatgcttta cgtgtgttta gtcgaggtta aaaaacgtct aggccccccg 2221 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatgattg aacaagatgg 2281 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 2341 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 2401 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 2461 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 2521 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 2581 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 2641 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 2701 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 2761 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 2821 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 2881 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 2941 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 3001 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 3061 gggactctgg ggttcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat 3121 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg 3181 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc 3241 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 3301 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc 3361 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga 3421 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 3481 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 3541 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 3601 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 3661 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 3721 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 4921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 4981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 5281 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca 5341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 5401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 5461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 5521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5581 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 5641 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 5701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 5761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 5821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 5881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 5941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 6061 tttcgtcttc aa //
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