LOCUS SYNMMLPLN4 5874 bp DNA SYN 28-FEB-1994 DEFINITION Moloney murine leukemia virus retroviral vector pLXSN, complete genome. ACCESSION M28248 NID g208848 KEYWORDS genome; neomycin phosphotransferase; retroviral vector. SOURCE Moloney murine leukemia virus DNA, Moloney murine sarcoma virus DNA, simian virus 40 DNA, transposon Tn5 DNA. ORGANISM unidentified cloning vector artificial sequence; cloning vectors. REFERENCE 1 (bases 1 to 5874) AUTHORS Miller,A.D. JOURNAL Unpublished (1989) REFERENCE 2 (sites) AUTHORS Miller,A.D. and Rosman,G.J. TITLE Improved retroviral vectors for gene transfer and expression JOURNAL BioTechniques 7, 980-990 (1989) MEDLINE 90211945 REFERENCE 3 (bases 1 to 5874) AUTHORS Miller,A.D. TITLE Direct Submission JOURNAL Submitted (22-SEP-1989) A.D. Miller, Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA FEATURES Location/Qualifiers source 1..5874 /organism="unidentified cloning vector" /db_xref="taxon:45196" LTR 175..763 /note="Mo-MuSV 5' long terminal repeat" misc_recomb 174^175 /note="mouse DNA end/Mo-MuSV DNA start" misc_signal 833..1642 /note="extended packaging signal" misc_recomb 1163^1164 /note="Mo-MuSV DNA end/Mo-MuLV DNA start" mutation 1223..1225 /note="gag ATG start codon to TAG stop codon" misc_recomb 1654^1655 /note="Mo-MuLV DNA end/simian virus 40 DNA start" misc_signal 1655..2020 /note="simian virus 40 early promoter" misc_recomb 2020^2021 /note="simian virus DNA end/Tn5 DNA start" CDS 2066..2860 /note="neomycin phosphotransferase" /codon_start=1 /transl_table=11 /db_xref="PID:g208849" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_recomb 2877^2878 /note="Tn5 DNA end/ Mo-MuLV DNA start" LTR 2920..3513 /note="Mo-MuLV 3' long terminal repeat" misc_recomb 3582^3583 /note="Mo-MuLV DNA end_plasmid pBR322 DNA start" BASE COUNT 1351 a 1611 c 1515 g 1397 t ORIGIN Junction between plasmid pBR322 and mouse genomic sequences. 1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa 181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa 241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata 301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag 361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg 421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca 481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca 541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc 601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat 661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg 721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga 781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta 901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac 961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc 1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt 1681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 1741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 1861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 1921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 1981 tttttggagg cctaggcttt tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca 2041 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 2101 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 2161 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 2221 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 2281 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 2341 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 2401 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 2461 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 2521 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 2581 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 2641 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 2701 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 2761 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 2821 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgat 2881 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag 2941 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag 3001 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag 3061 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga 3121 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac 3181 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc 3241 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc 3301 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct 3361 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa 3421 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag 3481 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc 3541 cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg 3601 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 3661 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 3721 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 3781 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 3841 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 3901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 3961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 4021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 4081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 4141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 4201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 4261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 4381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 4441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 4501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 4561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 4621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 4681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 4741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 4801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 4861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 4921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 4981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 5041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 5101 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 5161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 5221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 5281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 5341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 5401 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 5461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 5521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 5581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 5641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 5701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 5761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 5821 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa //
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