LOCUS SYNMOV1 6374 bp DNA SYN 20-OCT-1992 DEFINITION Moloney murine leukemia virus retroviral vector pLXSHD, complete sequence. ACCESSION M64753 NID g208855 KEYWORDS cloning vector; histidinol dehydrogenase; retroviral vector. SOURCE Synthetic, DNA. ORGANISM unidentified cloning vector artificial sequence; cloning vectors. REFERENCE 1 (bases 1 to 6374) AUTHORS Stockshlaeder,M.A.R., Storb,R., Osborne,W.R.A. and Miller,A.D. TITLE L-histidinol provides effective selection of retrovirus-vector-transduced keratinocytes without impairing their proliferative potential JOURNAL Hum. Gene Ther. 2, 33-39 (1991) MEDLINE 91322161 FEATURES Location/Qualifiers source 1..6374 /organism="unidentified cloning vector" /db_xref="taxon:45196" LTR 175..763 /note="Mo-MuSV 5' long terminal repeat" misc_signal 833..1642 /note="extended packaging signal" mutation 1223..1225 /note="gag ATG start codon to TAG stop codon" promoter 1655..1994 /note="simian virus 40 early promoter" CDS 2004..3308 /EC_number="1.1.1.23" /codon_start=1 /transl_table=11 /product="histidinol dehydrogenase" /db_xref="PID:g208856" /translation="MSFNTLIDWNSCSPEQQRALLTRPAISASDSITRTVSDILDNVK TRGDDALREYSAKFDKTEVTALRVTPEEIAAAGARLSDELKQAMTAAVKNIETFHSAQ TLPPVDVETQPGVRCQQVTRPVSSVGLYIPGGSAPLFSTVLMLATPARIAGCQKVVLC SPPPIADEILYAAQLCGVQEIFNVGGAQAIAALAFGSESVPKVDKIFGPGNAFVTEAK RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD IARKVAEAVERQLAELPRADTARQALSASRLIVTKDLAQCVAISNQYGPEHLIIQTRN ARDLVDAITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV QELSKAGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA" LTR 3420..4013 /note="Mo-MuLV 3' long terminal repeat" BASE COUNT 1482 a 1780 c 1628 g 1484 t ORIGIN 1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa 181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa 241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata 301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag 361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg 421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca 481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca 541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc 601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat 661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg 721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga 781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta 901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac 961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc 1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt 1681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 1741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 1861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 1921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 1981 tttttggagg cagatcccgg accatgagct tcaataccct gattgactgg aacagctgta 2041 gccctgaaca gcagcgtgcg ctgctgacgc gtccggcgat ttccgcctct gacagtatta 2101 cccggacggt cagcgatatt ctggataatg taaaaacgcg cggtgacgat gccctgcgtg 2161 aatacagcgc taaatttgat aaaacagaag tgacagcgct acgcgtcacc cctgaagaga 2221 tcgccgccgc cggcgcgcgt ctgagcgacg aattaaaaca ggcgatgacc gctgccgtca 2281 aaaatattga aacgttccat tccgcgcaga cgctaccgcc tgtagatgtg gaaacccagc 2341 caggcgtgcg ttgccagcag gttacgcgtc ccgtctcgtc tgtcggtctg tatattcccg 2401 gcggctcggc tccgctcttc tcaacggtgc tgatgctggc gacgccggcg cgcattgcgg 2461 gatgccagaa ggtggttctg tgctcgccgc cgcccatcgc tgatgaaatc ctctatgcgg 2521 cgcaactgtg tggcgtgcag gaaatcttta acgtcggcgg cgcgcaggcg attgccgctc 2581 tggccttcgg cagcgagtcc gtaccgaaag tggataaaat ttttggcccc ggcaacgcct 2641 ttgtaaccga agccaaacgt caggtcagcc agcgtctcga cggcgcggct atcgatatgc 2701 cagccgggcc gtctgaagta ctggtgatcg cagacagcgg cgcaacaccg gatttcgtcg 2761 cttctgacct gctctcccag gctgagcacg gcccggattc ccaggtgatc ctgctgacgc 2821 ctgatgctga cattgcccgc aaggtggcgg aggcggtaga acgtcaactg gcggaactgc 2881 cgcgcgcgga caccgcccgg caggccctga gcgccagtcg tctgattgtg accaaagatt 2941 tagcgcagtg cgtcgccatc tctaatcagt atgggccgga acacttaatc atccagacgc 3001 gcaatgcgcg cgatttggtg gatgcgatta ccagcgcagg ctcggtattt ctcggcgact 3061 ggtcgccgga atccgccggt gattacgctt ccggaaccaa ccatgtttta ccgacctatg 3121 gctatactgc tacctgttcc agccttgggt tagcggattt ccagaaacgg atgaccgttc 3181 aggaactgtc gaaagcgggc ttttccgctc tggcatcaac cattgaaaca ttggcggcgg 3241 cagaacgtct gaccgcccat aaaaatgccg tgaccctgcg cgtaaacgcc ctcaaggagc 3301 aagcatgagc actgaaaaca ctctcagcgt cgctgactta gcccgggatc tgcctaggct 3361 tttgcaaaaa gcttatcgat aaaataaaag attttattta gtctccagaa aaagggggga 3421 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca 3481 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca 3541 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca 3601 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 3661 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta 3721 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt 3781 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa 3841 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg 3901 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc 3961 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc 4021 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct 4081 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 4141 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 4201 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 4261 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 4321 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 4381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4981 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 5041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 5641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 5941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 6001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6301 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 6361 cctttcgtct tcaa //
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