LOCUS SYNMOV2 6837 bp DNA SYN 20-OCT-1992 DEFINITION Moloney murine leukemia virus retroviral vector pLHDCX, complete sequence. ACCESSION M64754 NID g208857 KEYWORDS cloning vector; histidinol dehydrogenase; retroviral vector. SOURCE Synthetic, DNA. ORGANISM unidentified cloning vector artificial sequence; cloning vectors. REFERENCE 1 (bases 1 to 6837) AUTHORS Stockshlaeder,M.A.R., Storb,R., Osborne,W.R.A. and Miller,A.D. TITLE L-histidinol provides effective selection of retrovirus-vector-transduced keratinocytes without impairing their proliferative potential JOURNAL Hum. Gene Ther. 2, 33-39 (1991) MEDLINE 91322161 FEATURES Location/Qualifiers source 1..6837 /organism="unidentified cloning vector" /db_xref="taxon:45196" LTR 175..763 /note="Mo-MuSV 5' long terminal repeat" misc_signal 833..1642 /note="extended packaging signal" mutation 1223..1225 /note="gag ATG start codon to TAG stop codon" CDS 1672..2976 /EC_number="1.1.1.23" /codon_start=1 /transl_table=11 /product="histidinol dehydrogenase" /db_xref="PID:g208858" /translation="MSFNTLIDWNSCSPEQQRALLTRPAISASDSITRTVSDILDNVK TRGDDALREYSAKFDKTEVTALRVTPEEIAAAGARLSDELKQAMTAAVKNIETFHSAQ TLPPVDVETQPGVRCQQVTRPVSSVGLYIPGGSAPLFSTVLMLATPARIAGCQKVVLC SPPPIADEILYAAQLCGVQEIFNVGGAQAIAALAFGSESVPKVDKIFGPGNAFVTEAK RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD IARKVAEAVERQLAELPRADTARQALSASRLIVTKDLAQCVAISNQYGPEHLIIQTRN ARDLVDAITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV QELSKAGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA" promoter 3017..3834 /note="human cytomegalovirus early promoter" LTR 3883..4476 /note="Mo-MuLV 3' long terminal repeat" BASE COUNT 1610 a 1877 c 1722 g 1628 t ORIGIN 1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa 181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa 241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata 301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag 361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg 421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca 481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca 541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc 601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat 661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg 721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga 781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta 901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac 961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc 1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatcccggac catgagcttc 1681 aataccctga ttgactggaa cagctgtagc cctgaacagc agcgtgcgct gctgacgcgt 1741 ccggcgattt ccgcctctga cagtattacc cggacggtca gcgatattct ggataatgta 1801 aaaacgcgcg gtgacgatgc cctgcgtgaa tacagcgcta aatttgataa aacagaagtg 1861 acagcgctac gcgtcacccc tgaagagatc gccgccgccg gcgcgcgtct gagcgacgaa 1921 ttaaaacagg cgatgaccgc tgccgtcaaa aatattgaaa cgttccattc cgcgcagacg 1981 ctaccgcctg tagatgtgga aacccagcca ggcgtgcgtt gccagcaggt tacgcgtccc 2041 gtctcgtctg tcggtctgta tattcccggc ggctcggctc cgctcttctc aacggtgctg 2101 atgctggcga cgccggcgcg cattgcggga tgccagaagg tggttctgtg ctcgccgccg 2161 cccatcgctg atgaaatcct ctatgcggcg caactgtgtg gcgtgcagga aatctttaac 2221 gtcggcggcg cgcaggcgat tgccgctctg gccttcggca gcgagtccgt accgaaagtg 2281 gataaaattt ttggccccgg caacgccttt gtaaccgaag ccaaacgtca ggtcagccag 2341 cgtctcgacg gcgcggctat cgatatgcca gccgggccgt ctgaagtact ggtgatcgca 2401 gacagcggcg caacaccgga tttcgtcgct tctgacctgc tctcccaggc tgagcacggc 2461 ccggattccc aggtgatcct gctgacgcct gatgctgaca ttgcccgcaa ggtggcggag 2521 gcggtagaac gtcaactggc ggaactgccg cgcgcggaca ccgcccggca ggccctgagc 2581 gccagtcgtc tgattgtgac caaagattta gcgcagtgcg tcgccatctc taatcagtat 2641 gggccggaac acttaatcat ccagacgcgc aatgcgcgcg atttggtgga tgcgattacc 2701 agcgcaggct cggtatttct cggcgactgg tcgccggaat ccgccggtga ttacgcttcc 2761 ggaaccaacc atgttttacc gacctatggc tatactgcta cctgttccag ccttgggtta 2821 gcggatttcc agaaacggat gaccgttcag gaactgtcga aagcgggctt ttccgctctg 2881 gcatcaacca ttgaaacatt ggcggcggca gaacgtctga ccgcccataa aaatgccgtg 2941 accctgcgcg taaacgccct caaggagcaa gcatgagcac tgaaaacact ctcagcgtcg 3001 ctgacttagc ccgggatccg gccattagcc atattattca ttggttatat agcataaatc 3061 aatattggct attggccatt gcatacgttg tatccatatc ataatatgta catttatatt 3121 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa 3181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 3241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 3301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 3361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 3421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 3481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 3541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 3601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 3661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc atgtacggtg ggaggtctat 3721 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 3781 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccaagct tgttaacatc 3841 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg 3901 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact 3961 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa 4021 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc 4081 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 4141 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt 4201 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt 4261 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc 4321 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat 4381 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct 4441 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag 4501 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt 4561 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 4621 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 4681 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 4741 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 4801 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 4861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 5041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 5101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 5221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 5461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 5581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 6001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 6061 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 6121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 6181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 6241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 6301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 6361 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 6421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 6781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa //
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