LOCUS SYNRRV 6259 bp DNA circular SYN 22-JUL-1993 DEFINITION Cloning vector pLXSH from Moloney murine leukemia virus, complete vector sequence. ACCESSION M77239 NID g209292 KEYWORDS expression vector; hygromycin phosphotransferase; retroviral vector. SOURCE Cloning vector DNA. ORGANISM unidentified cloning vector artificial sequence; cloning vectors. REFERENCE 1 (bases 1 to 6259) AUTHORS Miller,A.D., Miller,D.G., Garcia,J.V. and Lynch,C.M. TITLE Use of retroviral vectors for gene transfer and expression JOURNAL Meth. Enzymol. 217, 581-599 (1993) MEDLINE 93233494 FEATURES Location/Qualifiers source 1..6259 /organism="unidentified cloning vector" /note="Cloning vector pLXSH." /db_xref="taxon:45196" LTR 175..763 /note="Mo-MuSV 5' long terminal repeat" misc_signal 833..1642 /note="extended packaging signal" promoter 1655..2018 /note="simian virus 40 early promoter" CDS 2042..3079 /note="reporter gene (hygromycin phosphotransferase) from E.coli" /codon_start=1 /transl_table=11 /product="hygromycin phosphotransferase" /db_xref="PID:g209293" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRDQLGTGSHV" LTR 3305..3898 /note="Mo-MuLV 3' long terminal repeat" BASE COUNT 1449 a 1727 c 1607 g 1476 t ORIGIN 1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa 181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa 241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata 301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag 361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg 421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca 481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca 541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc 601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat 661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg 721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga 781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta 901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac 961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc 1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt 1681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 1741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 1861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 1921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 1981 tttttggagg cctaggcttt tgcaaaaagc tgcccaagct gatccccggg ggcaatgaga 2041 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga 2101 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga 2161 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga 2221 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat 2281 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt 2341 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga 2401 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg 2461 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta 2521 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga 2581 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg 2641 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc 2701 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc 2761 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc 2821 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga 2881 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg 2941 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg 3001 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtg atcagcttgg 3061 cactggttct catgtttgac agcttatcat cgcgaaatga ccgaccaagc gacgcccaac 3121 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 3181 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3241 gcccacccca acacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg 3301 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca 3361 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg 3421 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 3481 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 3541 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt 3601 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 3661 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 3721 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 3781 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 3841 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg 3901 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt 3961 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 4021 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 4081 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 4141 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 4201 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 4261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4861 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 5401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 5521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 5581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 5641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 5701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 5821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6181 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 6241 gaggcccttt cgtcttcaa //
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