ProtParam tool
User provided sequence:
MPCSGGNMKK SILFIFLSVL SFSPFAQDAK PVESSKEKIT LESKKCNIAK KSNKSGPESM
NSSNYCCELC CNPACTGCY
References and documentation are available.
Number of amino acids: 79
Molecular weight: 8555.9
Theoretical pI: 8.66
Amino acid composition:
Ala (A) 4 5.1%
Arg (R) 0 0.0%
Asn (N) 6 7.6%
Asp (D) 1 1.3%
Cys (C) 8 10.1%
Gln (Q) 1 1.3%
Glu (E) 5 6.3%
Gly (G) 4 5.1%
His (H) 0 0.0%
Ile (I) 4 5.1%
Leu (L) 5 6.3%
Lys (K) 10 12.7%
Met (M) 3 3.8%
Phe (F) 4 5.1%
Pro (P) 5 6.3%
Ser (S) 13 16.5%
Thr (T) 2 2.5%
Trp (W) 0 0.0%
Tyr (Y) 2 2.5%
Val (V) 2 2.5%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 6
Total number of positively charged residues (Arg + Lys): 10
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
-1 -1
Extinction coefficients are in units of M cm .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 4060 3816 3650 3520 3360
Abs 0.1% (=1 g/l) 0.475 0.446 0.427 0.411 0.393
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 2900 2800 2690 2560 2400
Abs 0.1% (=1 g/l) 0.339 0.327 0.314 0.299 0.281
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hour (mammalian reticulocytes, in vitro).
>20 hour (yeast, in vivo).
>10 hour (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 61.89
This classifies the protein as unstable.
Aliphatic index: 56.84
Grand average of hydropathicity (GRAVY): -0.241