表一 Published examples of heterologous protein expression in Pichia pastoris
Protein |
Expression Level (g/L) |
Where/How Expressed* |
Reference |
Angiotensin-conventing enzyme (90KD, active) |
n.r. |
S/Muts |
Sadhukhan, et al (1996) |
Dogfish shark cytochrome p450c17 (hydroxylase and lyase activity) |
n.r. |
I/Muts |
Trant, J.M. (1996) |
rabbit scFv (30kD) |
> 0.1g/L |
S/Muts |
Ridder, et al, (1995) |
Cryptosporidium parvum |
n.r. |
n.r. |
Jenkins and Fayer, (1995) |
human N-terminal transferrin |
0.27 |
S/Muts |
Steinlein, et al, (1995) |
amyloid precursor protein |
0.001 - 0.004 |
S/Muts |
Ohsawa, et al, (1995) |
human CD38 |
~0.16 |
S/Muts |
Fryxell, et al, (1995) |
Mouse OB gene (leptin) |
0.01 |
S/Mut+ |
Halaas, et al, (1995) |
Human MCP-3 (Monocyte Chemotactic Protein) |
0.001 |
S/Muts |
Masure, et al, (1995) |
Abbreviations
I = intracellular
Mut+ = methanol utilization wild type (genotype AOX1 AOX2)
Muts = methanol utilization slow (genotype aox1 AOX2)
S = secreted
n.r. = Information not reported or not available