表一 Published examples of heterologous protein expression in Pichia pastoris

 

 

Protein

Expression Level (g/L)

Where/How Expressed*

Reference

Angiotensin-conventing enzyme (90KD, active)

n.r.

S/Muts

Sadhukhan, et al (1996)

Dogfish shark cytochrome p450c17 (hydroxylase and lyase activity)

n.r.

I/Muts

Trant, J.M. (1996)

rabbit scFv (30kD)

> 0.1g/L

S/Muts

Ridder, et al, (1995)

Cryptosporidium parvum

n.r.

n.r.

Jenkins and Fayer, (1995)

human N-terminal transferrin

0.27

S/Muts

Steinlein, et al, (1995)

amyloid precursor protein

0.001 - 0.004

S/Muts

Ohsawa, et al, (1995)

human CD38

~0.16

S/Muts

Fryxell, et al, (1995)

Mouse OB gene (leptin)

0.01

S/Mut+

Halaas, et al, (1995)

Human MCP-3 (Monocyte Chemotactic Protein)

0.001

S/Muts

Masure, et al, (1995)

Abbreviations

I = intracellular

Mut+ = methanol utilization wild type (genotype AOX1 AOX2)

Muts = methanol utilization slow (genotype aox1 AOX2)

S = secreted

n.r. = Information not reported or not available