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ID M13 preliminary; circular DNA; PHG; 6407 BP.
XX
AC V00604; J02461; M10377;
XX
DT 15-MAR-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector M13 - complete.
XX
KW cloning vector.
XX
OS Bacteriophage M13
OC Viridae; ss-DNA nonenveloped viruses; Inoviridae.
XX
RN [1]
RP 1-6407
RC from M13, genes III/VI/I
RA Van Wezenbeek P.M., Schoenmakers J.G.;
RT "Nucleotide sequence of the genes III, VI and I of bacteriophage M13";
RL Nucleic Acids Res. 6:2799-2818(1979).
XX
RN [2]
RP 1-6407
RC from M13
RC from phage fd
RA Van Wezenbeek P.M., Hulsebos T.J., Schoenmakers J.G.;
RT "Nucleotide sequence of the filamentous bacteriophage M13 genome:
RT comparison with phage fd";
RL Gene 11:129-148(1980).
XX
RN [3]
RP 5725-5997
RC pJMC122 from pBR322 & M13, ori
RC pJMC67 from pBR322 & M13, ori
RA Cleary J.M., Ray D.S.;
RT "Deletion analysis of the cloned replication origin region from
RT bacteriophage M13";
RL J. Virol. 40:197-203(1981).
XX
RN [4]
RC M13
RA Mount D.;
RT "genet msg # 81; genet msg # 78 and 53";
RL Submitted (15-MAR-1988) to GenBank on sumex tape by:
RL Mount D., Univ. of Arizona.
XX
CC Sequence was compared to the sumex tape.
CC Known differences from Suggs & Ray are: 5538a (inserted),
CC 5558-5559ca (tc).
CC Replicative form is duplex.
CC Nucleotides 5725-5997 from the origin region of the M13 duplex
CC replicative form DNA confer an M13-dependent replication mechanism
CC upon the plasmid vector pBR322 [3].
CC This M13 insert permits M13 helper-dependent replication of the
CC hybrid plasmid in polA cells which are unable to replicate the
CC pBR322 replicon alone [3].
CC The segment required for high frequency transformation by oriM13
CC plasmids extends a maximum of 41 nucleotides beyond the HaeIII site
CC at positions 5867-5870, but does not include most of the 'at'-rich
CC DNA at the right hand end of the DNA [3].
CC NM (M13)
CC CM (yes)
CC NA (filamentous ss-DNA)(ds-DNA)
CC TP (linear)(circular)
CC ST ()
CC TY (phage)
CC SP (USB)
CC HO (E.coli)
CC CP ()
CC FN (cloning)(sequencing)
CC SE ()
CC PA (M13)
CC BR (fd)(f1)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli, ff phages
FT -> M13 6407bp"
FT CDS complement(6016..6407)
FT /note="GEN bacteriophage M13 gene II (across origin);
FT protein required for replication; putative"
FT CDS complement(1..818)
FT /note="GEN bacteriophage M13 gene II (across origin);
FT protein required for replication; putative"
FT CDS 496..831
FT /note="GEN bacteriophage M13 X gene (function
FT unknown)"
FT CDS 843..1106
FT /note="GEN bacteriophage M13 V gene (single-stranded
FT binding protein)"
FT CDS 1108..1209
FT /note="GEN bacteriophage M13 VII gene (morphogenesis)"
FT CDS 1206..1304
FT /note="GEN bacteriophage M13 IX gene (minor coat
FT protein)"
FT mRNA <1301..1551
FT /note="RNA mRNA (3' end +/- 14 bp)"
FT CDS 1301..1522
FT /note="GEN bacteriophage M13 VIII gene (major coat
FT protein)"
FT CDS 1579..2853
FT /note="GEN bacteriophage M13 III gene (adsorption
FT protein)"
FT CDS 2856..3194
FT /note="GEN bacteriophage M13 VI gene (morphogenesis)"
FT CDS 3196..4242
FT /note="GEN bacteriophage M13 I gene (morphogenesis)"
FT CDS 4220..5500
FT /note="GEN bacteriophage M13 IV gene (morphogenesis)"
FT misc_RNA <5725..5755
FT /note="RNA ori RNA [3]"
FT rep_origin 5781..5781
FT /note="ORI bacteriophage M13 origin of viral
FT strand synthesis"
XX
SQ Sequence 6407 BP; 1575 A; 1296 C; 1315 G; 2221 T; 0 other;
aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
tttaaatatt tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg
tacatatgat tgacatgcta gttttacgat taccgttcat cgattctctt gtttgctcca
gactctcagg caatgacctg atagcctttg tagacctctc aaaaatagct accctctccg
gcatgaattt atcagctaga acggttgaat atcatattga tggtgatttg actgtctccg
gcctttctca cccttttgaa tctttaccta cacattactc aggcattgca tttaaaatat
atgagggttc taaaaatttt tatccttgcg ttgaaataaa ggcttctccc gcaaaagtat
tacagggtca taatgttttt ggtacaaccg atttagcttt atgctctgag gctttattgc
ttaattttgc taattctttg ccttgcctgt atgatttatt ggatgtt
//