1. Sho-Ming cloned a gene in the lab. The DNA sequence is listed here:

AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAACCCTAAGGTCCTATA GTGTTCTATATCCAACACTAGCTCCTACTCCCTAAAGCATTTATTATATCTTCCCCT AGCTAGATACTTCATTCCACAAATAGTTTGCAGCTTTTTCTTTTCCTCTAAAACAAT GGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTGCATGGTGGTAGCTGCACC CTGCGCAGAAGCCATAACCTGTGGCCAGGTTACGTCGAATTTGGCACCTTGTCTTGC TTATCTTAGAAACACGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGT GAATTCTGCAAGGACCACAGAAGATCGTCAAATTGCATGCACTTGCCTGAAATCAGC TGCAGGTGCTATTTCTGGAATCAATTTGGGCAAAGCTGCTGGTCTCCCTAGTACTTG TGGTGTCAATATTCCTTACAAGATCAGCCCTTCCACTGACTGCTCCAAGTACCTCAC TTTTTTTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTTCATTTTCGCTT ATCTTTTAAATTTTTTATTCGGAATCTTTATACCA

Please help him to identify the gene and its protein sequence.

Ans:

tabacum ltp1 gene for lipid transferase. 3112 bp

Source: common tobacco.

Coding region: 1347--1681, 2662--2671

Sequence: 114 aa

MEIAGKIACFVVLCMVVAAPCAEAITCGQVTSNLAPCLAYLRNTGPLGRCCGGVKALVNSARTTEDRQIA CTCLKSAAGAISGINLGKAAGLPSTCGVNIPYKISPSTDCSKVQ

2. How many nucleotide and protein sequence of Lycopersicon esculentum were know? Please find its class II small heat shock protein mRNA, complete cds.

Ans:

1) Lycopersicon esculentum=>tomato

protein sequences:1019

nucleotide sequences: 742

polyA_signal: 629..634

BASE COUNT: 229 a 117 c 183 g 209 t

2) class II small heat shock protein mRNA complete cds:

1 tacggctgcg agaagacgac agaaggggac tgcaattaca aatcaaacca aaattgacaa

61 atttcacgca caaaatcaca atatccaaaa atttctcaat actgaaaatg gatttgaggt

121 tgttgggtat cgataacaca ccactcttcc acactctcca ccatatgatg gaagctgccg

181 gtgaagattc cgacaagtct gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg

241 ccatggctgc tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg

301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac aatgtgctgt

361 tgattagtgg tgaaaggaag agggaagaag agaaagaagg tgcaaagttt attaggatgg

421 agagaagggt tgggaaattc atgaggaagt ttagtctgcc agagaatgcg aatactgatg

481 caatttctgc agtttgtcaa gatggagttc tgactgttac tgttcagaaa ttgcctcctc

541 ctgagccaaa gaaacccaaa acaattgagg tgaaagttgc ttgaagttat ggactctgtt

601 ttgatggttt gtggtatgat gtagtagaaa taaagttgta ggagtagtga acttttcctt

661 tcatctttct gctatgtttt cacgtctgtt tgaatgttac aatagccatg ggtattgttt

721 gttttgatgc caaaaaaa

translation="MDLRLLGIDNTPLFHTLHHMMEAAGEDSDKSVNAPSRNYVRDAK AMAATPADVKEYPNSYVFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKFI RMERRVGKFMRKFSLPENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKVA"