Snake toxins signature
38-58 GCIDVCPESSLIVKYVCCNTD
(5)
(1)
Please help him to find its corresponding polypeptide sequence (DNA ->
Protein
translation).
(2)
Please help him to calculate pI/Mw of this polypeptide, perform the
trypsin
cutting , analyze the cutting pattern and report the fragments with
molecular
weight over 500 Dalton.
(3) Please help him to identify this toxin. Is it a new toxin?
(4)
Please help him to use Prosite scanning tool to find out possible
functions
or pattern of this polypeptide.
(5)
David would like to see its structure. Could you help him to find
structure
of this toxin or make a model if it is a new protein? Show structure
on
your homwpage ( 3 different views).
Ans:
(1) Met
L E C N E L I P L F Y E T C P A G E N I C Y E Met F Met V A T
P K V P C E R G C I D V C P E S S L I V K Y V C C N T D R C Q Stop
(2) Theoretical pI/Mw: 4.29 / 6917.13
MS-Digest Results :
Digestion enzyme: Trypsin
Cysteines modified by: unmodified
| Start | End | MH+(mi) | MH+(av) | sequence |
| 33 | 37 | 603.2925 | 603.7 | (K)VPCER(G) |
| 52 | 59 | 973.3827 | 974.1 | (K)YVCCNTDR(C) |
| 52 | 61 | 1204.4549 | 1205.4 | (K)YVCCNTDRCQ(-) |
| 38 | 51 | 1462.7286 | 1463.8 | (R)GCIDVCPESSLIVK(Y) |
| 33 | 51 | 2047.0026 | 2048.5 | (K)VPCERGCIDVCPESSLIVK(Y) |
| 38 | 59 | 2417.0973 | 2418.8 | (R)GCIDVCPESSLIVKYVCCNTDR(C) |
| 38 | 61 | 2648.1651 | 2650.1 | (R)GCIDVCPESSLIVKYVCCNTDRCQ(-) |
| 33 | 59 | 3001.3714 | 3003.5 | (K)VPCERGCIDVCPESSLIVKYVCCNTDR(C) |
| 1 | 32 | 3699.6627 | 3702.4 | (-)MLECNELIPLFYETCPAGENICYEMFMVATPK(V) |
| 1 | 37 | 4283.9368 | 4287.1 | (-)MLECNELIPLFYETCPAGENICYEMFMVATPKVPCER(G) |
| 1 | 51 | 5727.6470 | 5731.9 | (-)MLECNELIPLFYETCPAGENICYEMFMVATPKVPCERGCIDVCPESSLIVK(Y) |
(4)1. PDOC00005 PS00005 PKC_PHOSPHO_SITE
Protein
kinase C phosphorylation site
Number of matches: 2
1
30-32 TPK
2
57-59 TDR
2. PDOC00245 PS00272 SNAKE_TOXIN
Snake
toxins signature
38-58 GCIDVCPESSLIVKYVCCNTD
(5)