SAPS. Version of January 7, 1995. Date run: Tue Dec 16 12:48:44 1997 SWISS-PROT ANNOTATION: ID 27440 DE 27440, 60 bases, 1E82 checksum. number of residues: 60; molecular weight: 6.8 kdal 1 LKCNKLVPLF YKTCPAGKNI CYKMFMVATP KLPVKRGCID VCPKSSLLVR YVCCNTDKCN COMPOSITIONAL ANALYSIS (extremes relative to: swp23s.q) A : 2( 3.3%); C : 8(13.3%); D : 2( 3.3%); E : 0( 0.0%); F : 2( 3.3%) G : 2( 3.3%); H : 0( 0.0%); I : 2( 3.3%); K : 9(15.0%); L : 6(10.0%) M : 2( 3.3%); N : 4( 6.7%); P : 5( 8.3%); Q : 0( 0.0%); R : 2( 3.3%) S : 2( 3.3%); T : 3( 5.0%); V : 6(10.0%); W : 0( 0.0%); Y : 3( 5.0%) KR : 11 ( 18.3%); ED : 2 ( 3.3%); AGP : 9 ( 15.0%); KRED : 13 ( 21.7%); KR-ED : 9 ( 15.0%); FIKMNY : 22 ( 36.7%); LVIFM : 18 ( 30.0%); ST : 5 ( 8.3%). CHARGE DISTRIBUTIONAL ANALYSIS 1 0+00+00000 0+00000+00 00+0000000 +000++000- 000+00000+ 000000-+00 A. CHARGE CLUSTERS. Positive charge clusters (cmin = 13/30 or 18/45 or 22/60): none Negative charge clusters: not evaluated (frequency of - < 5%, too low) Mixed charge clusters (cmin = 15/30 or 20/45 or 25/60): none B. HIGH SCORING (UN)CHARGED SEGMENTS. High scoring positive charge segments: score= 2.00 frequency= 0.183 ( KR ) score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.783 ( LAGSVTIPNFQYHMCW ) score= -2.00 frequency= 0.033 ( ED ) Expected score/letter: -0.483 - now scoring for positive charge segments; Average information/letter: 0.430 Minimal length of displayed segments set to: 20 M_0.01= 13.07 (cv= 7.77, lambda= 0.52686, k= 0.16371, x= 5.30; 90% confidence interval for segment length: 23 +- 25) M_0.05= 9.97 (x= 2.20) # of segments (>=20 residues) exceeding M_0.05: none High scoring negative charge segments: score= 2.00 frequency= 0.033 ( ED ) score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.783 ( LAGSVTIPNFQYHMCW ) score= -2.00 frequency= 0.183 ( KR ) Expected score/letter: -1.083 - now scoring for negative charge segments; Average information/letter: 3.490 Minimal length of displayed segments set to: 20 M_0.01= 4.94 (cv= 2.54, lambda= 1.61122, k= 0.47671, x= 2.40; 90% confidence interval for segment length: 3 +- 3) M_0.05= 3.92 (x= 1.38) # of segments (>=20 residues) exceeding M_0.05: none High scoring mixed charge segments: score= 1.00 frequency= 0.217 ( KEDR ) score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.783 ( LAGSVTIPNFQYHMCW ) Expected score/letter: -0.567 - now scoring for mixed charge segments; Average information/letter: 1.051 Minimal length of displayed segments set to: 20 M_0.01= 6.07 (cv= 3.19, lambda= 1.28520, k= 0.40993, x= 2.89; 90% confidence interval for segment length: 11 +- 9) M_0.05= 4.80 (x= 1.62) # of segments (>=20 residues) exceeding M_0.05: none High scoring uncharged segments: score= 1.00 frequency= 0.783 ( LAGSVTIPNFQYHMCW ) score= 0.00 frequency= 0.000 ( BZX ) score= -8.00 frequency= 0.217 ( KEDR ) Expected score/letter: -0.950 - now scoring for uncharged segments; Average information/letter: 0.173 Minimal length of displayed segments set to: 20 M_0.01= 32.53 (cv= 20.56, lambda= 0.19916, k= 0.10900, x= 11.97; 90% confidence interval for segment length: 54 +- 44) M_0.05= 24.34 (x= 3.79) # of segments (>=20 residues) exceeding M_0.05: none C. CHARGE RUNS AND PATTERNS. pattern (+)| (-)| (*)| (0)| (+0)| (-0)| (*0)|(+00)|(-00)|(*00)| lmin0 5 | 3 | 6 | 29 | 10 | 6 | 10 | 11 | 6 | 12 | lmin1 6 | 4 | 7 | 36 | 12 | 7 | 12 | 14 | 8 | 14 | lmin2 7 | 4 | 8 | 39 | 13 | 8 | 14 | 15 | 9 | 16 | There are no charge runs or patterns exceeding the given minimal lengths. Run count statistics: + runs >= 3: 0 - runs >= 3: 0 * runs >= 4: 0 0 runs >= 20: 0 DISTRIBUTION OF OTHER AMINO ACID TYPES 1. HIGH SCORING SEGMENTS. High scoring hydrophobic segments: 2.00 (LVIFM) 1.00 (AGYCW) 0.00 (BZX) -2.00 (PH) -4.00 (STNQ) -8.00 (KEDR) Expected score/letter: -1.650 - now scoring for hydrophobic segments ........40........80.......120.......160.......200 ******; Average information/letter: 0.452 Minimal length of displayed segments set to: 15 M_0.01= 23.51 (cv= 13.41, lambda= 0.30526, k= 0.21941, x= 10.10; 90% confidence interval for segment length: 23 +- 17) M_0.05= 18.17 (x= 4.76) # of segments (>=15 residues) exceeding M_0.05: none High scoring transmembrane segments: 5.00 (LVIF) 2.00 (AGM) 0.00 (BZX) -1.00 (YCW) -2.00 (ST) -6.00 (P) -8.00 (H) -10.00 (NQ) -16.00 (KR) -17.00 (ED) Expected score/letter: -3.483 - now scoring for transmembrane segments ........40........80.......120.......160.......200 ******; Average information/letter: 0.513 Minimal length of displayed segments set to: 15 M_0.01= 45.47 (cv= 26.27, lambda= 0.15585, k= 0.20016, x= 19.19; 90% confidence interval for segment length: 20 +- 16) M_0.05= 35.01 (x= 8.74); M_0.30= 22.56 (x= -3.71) # of segments (>=15 residues) exceeding M_0.30: none 2. SPACINGS OF C. H2N-2-C-10-C-6-C-16-C-3-C-10 CC at 53 -4-C-1-COOH REPETITIVE STRUCTURES. A. SEPARATED, TANDEM, AND PERIODIC REPEATS: amino acid alphabet. Repeat core block length: 4 B. SEPARATED AND TANDEM REPEATS: 11-letter reduced alphabet. (i= LVIF; += KR; -= ED; s= AG; o= ST; n= NQ; a= YW; p= P; h= H; m= M; c= C) Repeat core block length: 8 MULTIPLETS. A. AMINO ACID ALPHABET. 1. Total number of amino acid multiplets: 3 (Expected range: 0-- 11) 2. Histogram of spacings between consecutive amino acid multiplets: (1-5) 2 (6-10) 1 (11-20) 0 (>=21) 1 3. Clusters of amino acid multiplets (cmin = 10/30 or 12/45 or 15/60): none B. CHARGE ALPHABET. 1. Total number of charge multiplets: 1 (Expected range: 0-- 6) 1 +plets (f+: 18.3%), 0 -plets (f-: 3.3%) Total number of charge altplets: 1 (Critical number: 4) 2. Histogram of spacings between consecutive charge multiplets: (1-5) 0 (6-10) 0 (11-20) 0 (>=21) 2 PERIODICITY ANALYSIS. A. AMINO ACID ALPHABET (core: 4; !-core: 4) Location Period Element Copies Core Errors There are no periodicities of the prescribed length. B. CHARGE ALPHABET ({+= KR; -= ED; 0}; core: 5; !-core: 5) and HYDROPHOBICITY ALPHABET ({*= KRED; i= LVIF; 0}; core: 6; !-core: 8) Location Period Element Copies Core Errors There are no periodicities of the prescribed length. SPACING ANALYSIS. Not evaluated (sequence length < 100 aa, too short).