HOMEWORK#5

1. Sho-Ming cloneda gene in the lab. The DNA sequence is listed here:

AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAACCCTAAGGTCCTATA

GTGTTCTATATCCAACACTAGCTCCTACTCCCTAAAGCATTTATTATATCTTCCCCT

AGCTAGATACTTCATTCCACAAATAGTTTGCAGCTTTTTCTTTTCCTCTAAAACAAT

GGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTGCATGGTGGTAGCTGCACC

CTGCGCAGAAGCCATAACCTGTGGCCAGGTTACGTCGAATTTGGCACCTTGTCTTGC

TTATCTTAGAAACACGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGT

GAATTCTGCAAGGACCACAGAAGATCGTCAAATTGCATGCACTTGCCTGAAATCAGC

TGCAGGTGCTATTTCTGGAATCAATTTGGGCAAAGCTGCTGGTCTCCCTAGTACTTG

TGGTGTCAATATTCCTTACAAGATCAGCCCTTCCACTGACTGCTCCAAGTACCTCAC

TTTTTTTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTTCATTTTCGCTT

ATCTTTTAAATTTTTTATTCGGAATCTTTATACCA

2. How many nucleotide and protein sequence of Lycopersicon esculentum were know?

1.Ans : 


(1)DEFINITION : N.tabacum ltp1 gene for lipid transferase. 

(2)LOCUS      : NTLTP1       3112 bp    DNA

(3)SOURCE     : common tobacco.

(4)ORGANISM   : Nicotiana tabacum  

(5)PROTEIN SEQUENCE :
               
   MEIAGKIACF VVLCMVVAAP CAEAITCGQV TSNLAPCLAY LRNTGPLGRC 
   CGGVKALVNS ARTTEDRQIA CTCLKSAAGA ISGINLGKAA GLPSTCGVNI
   PYKISPSTDC SKVQ

2.Ans : 


(1) 744 nucleotide sequences were known.

    1021 protein sequences were known.

(2) Lycopersicon esculentum class II small heat shock protein mRNA :

     1 tacggctgcg agaagacgac agaaggggac tgcaattaca aatcaaacca aaattgacaa
    61 atttcacgca caaaatcaca atatccaaaa atttctcaat actgaaaatg gatttgaggt
   121 tgttgggtat cgataacaca ccactcttcc acactctcca ccatatgatg gaagctgccg
   181 gtgaagattc cgacaagtct gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg
   241 ccatggctgc tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg
   301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac aatgtgctgt
   361 tgattagtgg tgaaaggaag agggaagaag agaaagaagg tgcaaagttt attaggatgg
   421 agagaagggt tgggaaattc atgaggaagt ttagtctgcc agagaatgcg aatactgatg
   481 caatttctgc agtttgtcaa gatggagttc tgactgttac tgttcagaaa ttgcctcctc
   541 ctgagccaaa gaaacccaaa acaattgagg tgaaagttgc ttgaagttat ggactctgtt
   601 ttgatggttt gtggtatgat gtagtagaaa taaagttgta ggagtagtga acttttcctt
   661 tcatctttct gctatgtttt cacgtctgtt tgaatgttac aatagccatg ggtattgttt
   721 gttttgatgc caaaaaaa