ProtParam tool

User provided sequence:
MDPRKVSELR AFVKMCRQDP SVLHTEEMRF LREWVESMGG KVPPATHKAK SEENTKEEKR 
DKTTEDNIKT EEPSSEESDL EIDNEGVIEA DTDAPQEMGD ENAEITEAMM DEANEKKGAA 
IDALNDGELQ KAIDLFTDAI KLNPRLAILY AKRASVFVKL QKPNAAIRDC DRAIEINPDS 
AQPYKWRGKA HRLLGHWEEA ARDLALACKL DYDEDASAML REVQPRAQKI AEHRRKYERK 
REEREIKERI ERVKKAREEH EKAQREEEAR RQSGSQFGSF PGGFPGGMPG NFPGGMPGMG 
GAMPGMAGMP GLNEILSDPE VLAAMQDPEV MVAFQDVAQN PSNMSKYQNN PKVMNLISKL 
SAKFGGHS

Number of amino acids: 368

Molecular weight: 41279.5

Theoretical pI: 5.28

Amino acid composition:

Ala (A)  39	 10.6%
Arg (R)  26	  7.1%
Asn (N)  16	  4.3%
Asp (D)  24	  6.5%
Cys (C)   3	  0.8%
Gln (Q)  14	  3.8%
Glu (E)  45	 12.2%
Gly (G)  25	  6.8%
His (H)   7	  1.9%
Ile (I)  16	  4.3%
Leu (L)  22	  6.0%
Lys (K)  30	  8.2%
Met (M)  18	  4.9%
Phe (F)  10	  2.7%
Pro (P)  22	  6.0%
Ser (S)  19	  5.2%
Thr (T)   9	  2.4%
Trp (W)   3	  0.8%
Tyr (Y)   5	  1.4%
Val (V)  15	  4.1%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 56


Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

                                         -1   -1
Extinction coefficients are in units of M   cm .

The first table lists values computed assuming ALL Cys 
residues appear as half cysteines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    23885   24181   24065   23830   23160
Abs 0.1% (=1 g/l)   0.579   0.586   0.583   0.577   0.561



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    23450   23800   23705   23470   22800
Abs 0.1% (=1 g/l)   0.568   0.577   0.574   0.569   0.552


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hour (mammalian reticulocytes, in vitro).
                            >20 hour (yeast, in vivo).
                            >10 hour (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.06
This classifies the protein as unstable.



Aliphatic index: 62.69

Grand average of hydropathicity (GRAVY): -0.870