- Collect Bio-related database and add to your bookmarks.
- Pick up your favorite one. Give me its address and a short description.
- Give me an example that shows -
- (1) How you get the information from this database.
- (2) What information you get from this database.
- This homework should be submitted in your homepage.
ExPASy
Molecular Biological Server :
#TOOLS - Access to many protein analysis tools
#Compute pI/Mw - Compute the theoretical pI and Mw from a SWISS-PROT or TREMBL entry or for a user sequence
*address : http://expasy.hcuge.ch/ch2d/pi_tool.html
- This tool calculates the estimated pI and MW of a specified SWISS-PROT entry or a user-entered AA sequence. These parameters are useful if you want to know the approximate region of a 2-D gel where a protein may be found.
- To use the program, enter one or more SWISS-PROT identification names (e.g. LACB_BOVIN) or accession numbers (e.g. P02754) into the text field, and select the "click here to compute pI/Mw" button. If the entries you specified have features described in the database, you will be asked to select one of the areas of the protein displayed as a hypertext link, or numerically specify the region of interest. Alternatively, if your protein of interest is not in the SWISS-PROT database, you can enter an AA sequence in standard single letter AA code into the text field, and select the "click here to compute pI/Mw" button. The predicted pI and MW of your sequence will then be displayed. A typical output from the program is shown in
*enter one or more SWISS-PROT protein identifiers (ID) (e.g. ALBU_HUMAN) or SWISS-PROT/TREMBL accession numbers (AC) (e.g. P04406), separated by spaces, tabs or newlines. Alternatively, enter a protein sequence in single letter code. The theoretical pI and Mw (molecular weight) will then be computed.
*I want to find the pI and Mw of the Bacteriophage Lambda Cro Repressor
- SWISS-PROT: P03040
- ID : RCRO_LAMBD
- protein squence : MEQRITLKDY AMRFGQTKTA KDLGVYQSAI NKAIHAGRKI FLTINADGSV YAEEVKPFPS NKKTTA
*enter protein sequence
*result:
Theoretical pI/Mw for the protein sequence
MEQRITLKDY ... PFPSNKKTTA :
Theoretical pI/Mw: 9.75 / 7363.48