HOMEWORK#4

Molecular viewer

Find an interesting protein, say, subtilisin, and give a brief description of the structure and function of this protein. And then use Rasmol to show the structural features of this protein. You should put in your homepage your Rasmol scripts as plain text and some of the represenative images generated by the script.

Answer :

<1AF4 : Serine Protease; date : Mar 21, 1997; title : Crystal Structure Of Subtilisin Carlsberg In Anhydrous Dioxane; authors : J.L.Schmitke, L.J.Stern, A.M.Klibanov>

*Fuction : Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of prpteins and peptide amides.

*Catalytic activity : Hydrolysis of proteins with broad specificty for peptide bonds, and a preference for a large uncharged residue in p1. hydrolyses peptide amides.

*Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

*Similarity : Belongs to peptidase family S8; also known as the subtilase family. 

*Sequence : 379 AA; 38908 MW;

MMRKKSFWLG MLTAFMLVFT MAFSDSASAA QPAKNVEKDY IVGFKSGVKT ASVKKDIIKE SGGKVDKQFR IINAAKAKLD KEALKEVKND PDVAYVEEDH VAHALAQTVP YGIPLIKADK VQAQGFKGAN VKVAVLDTGI QASHPDLNVV GGASFVAGEA YNTDGNGHGT HVAGTVAALD NTTGVLGVAP SVSLYAVKVL NSSGSGTYSG IVSGIEWATT NGMDVINMSL GGPSGSTAMK QAVDNAYARG VVVVAAAGNS GSSGNTNTIG YPAKYDSVIA VGAVDSNSNR ASFSSVGAEL EVMAPGAGVY STYPTSTYAT LNGTSMASPH VAGAAALILS KHPNLSASQV RNRLSSTATY LGSSFYYGKG LINVEAAAQ

*Structure :

Rasmol scripts :