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The answers of  questions 1 are solved and reconstituted as the table

Questions 1

a.) PDB ID number.  b.) Name of the authors.  c.) Expression system used by authors. 
d.) Residue number. e.) Number of helix. f.) The starting residue and ending residue of each helix. 
g.) Number of b-sheet.
This is 1EMA
1EMA Fluorescent Protein  date Aug 01, 1996
title  Green Fluorescent Protein From Aequorea Victoria
authors  M.Ormo, A.B.Cubitt, K.Kallio, L.A.Gross, R.Y.Tsien, S.J.Remington
compound source
Molecule: Green Fluorescent Protein
Chain: Null
Engineered: Yes
Mutation: 65 - 67 Replaced By Cro, Q80r
Other_details: The Protein Contains Six Se-Methionines. Of These, The N-Terminal Met And Met 233 Are Not Present In The Entry		 Organism_scientific: Aequorea Victoria
Organism_common: Jellyfish
Plasmid: Prsetb (Invitrogen)
Expression_system: Escherichia Coli
Expression_system_strain: Jm109 (De3)
Other_details: The N-Terminal His-Tag Has Been Removed
symmetry  Space Group: P 21 21 21 R_factor 
crystal
cell
length a length b length c angle alpha angle beta angle gamma
51.770 62.850 70.670 90.00 90.00 90.00
 
method  X-Ray Diffraction resolution  1.9 Å
ligand  CRO, MSE
number of helix 4 residue number 236
number of b-sheet 11
helix start and stop
Helix 1: From GLY 4 to PHE 8
Helix 2: From TRP 57 to LEU 60
Helix 3: From GIN 69 to PHE 71
Helix 4: From PHE 83 to SER 86
Questions 2
Show three different Rasmol pictures of GFP: 
  whatever you like. 
  highlight the chromophore by spacefill and green. 
  a rotating molecule by GIF builder.


Questions 3
A blue emission variant of GFP has been made by mutation of two residues. Please find the structure
of this blue emission variant. Show its structure and color the two mutated positions blue.





The mutation site : Y66H and Y145F








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