HOMEWORK#3
Average images from GroEL side views in the absenceorpresence of ADP and GroES. Escherichia coli GroEL and GroES are the prototypical chaperonin and co-chaperonin. Chaperonins arering-shaped molecular chaperone proteins that are found in all organisms and are essential for protein folding and protection from stress. Both the chaperonin and co-chaperonin are 7-fold symmetric oligomers. GroEL is composed of fourteen identical 60 kDa subunits arranged in two stacked rings, and GroES is composed of a seven identical 10 kDa subunits.

1. Show the crystal structure of a single GroES subunit.

  • restrict carbon
  • strabds
  • restrict :O
  • color structure
  • zoom 250

    • 2. Show the crystal structure of a single GroEL subunit.

  • restrict carbon
  • strands
  • restrict :a
  • color structure
  • zoom 250

    • 3. Show "top view" C-alpha trace colored by chain of GroEL
    and measure the width of its central cavities.

  • restrict carbon
  • backbone 0.3
  • restrict :a or :b or :c or :d or :e or :f or :g
  • select :*
  • color *
  • set picking distance
  • zoom 160

    • 4. Show "side view" cartoon display colored by structure
    of GroES and measure its width.

  • restrict carbon
  • cartoons
  • restrict :o or :p or :q or :r or :s or :t or :u
  • color structure
  • center glu50:t
  • rotate x 270
  • zoon 200
  • set picking distance
  • color labels blue

    • 5. Estimate the central cavities' width of GroEL from image shown above. Compare this width with the one you measured from crystal structure.

      Ans:

           The width of above images is 5.0 nm,
     
           It is 4.5nm in width of crystal structure my measured.