HOMEWORK#4
1.Please find the genes for GroEL and GroES
that you showedthe structures in homework 3.
GroEs:2178..2471 color=green
GroEl:2515..4161 color=blue
>gi|1790582|gb|AE000487|AE000487 Escherichia coli K-12 MG1655
section 377 of 400 of the complete genome
2101 tggtcaccag ccgggaaacc acgtaagctc cggcgtcacc cataacagat acggactttc
2161 tcaaaggaga gttatcaatg aatattcgtc cattgcatga tcgcgtgatc gtcaagcgta
2221 aagaagttga aactaaatct gctggcggca tcgttctgac cggctctgca gcggctaaat
2281 ccacccgcgg cgaagtgctg gctgtcggca atggccgtat ccttgaaaat ggcgaagtga
2341 agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac ggtgtgaaat
2401 ctgagaagat cgacaatgaa gaagtgttga tcatgtccga aagcgacatt ctggcaattg
2461 ttgaagcgta atccgcgcac gacactgaac atacgaattt aaggaataaa gataatggca
2521 gctaaagacg taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg cgtaaacgta
2581 ctggcagatg cagtgaaagt taccctcggt ccaaaaggcc gtaacgtagt tctggataaa
2641 tctttcggtg caccgaccat caccaaagat ggtgtttccg ttgctcgtga aatcgaactg
2701 gaagacaagt tcgaaaatat gggtgcgcag atggtgaaag aagttgcctc taaagcaaac
2761 gacgctgcag gcgacggtac caccactgca accgtactgg ctcaggctat catcactgaa
2821 ggtctgaaag ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg tatcgacaaa
2881 gcggttaccg ctgcagttga agaactgaaa gcgctgtccg taccatgctc tgactctaaa
2941 gcgattgctc aggttggtac catctccgct aactccgacg aaaccgtagg taaactgatc
3001 gctgaagcga tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga cggtaccggt
3061 ctgcaggacg aactggacgt ggttgaaggt atgcagttcg accgtggcta cctgtctcct
3121 tacttcatca acaagccgga aactggcgca gtagaactgg aaagcccgtt catcctgctg
3181 gctgacaaga aaatctccaa catccgcgaa atgctgccgg ttctggaagc tgttgccaaa
3241 gcaggcaaac cgctgctgat catcgctgaa gatgtagaag gcgaagcgct ggcaactctg
3301 gttgttaaca ccatgcgtgg catcgtgaaa gtcgctgcgg ttaaagcacc gggcttcggc
3361 gatcgtcgta aagctatgct gcaggatatc gcaaccctga ctggcggtac cgtgatctct
3421 gaagagatcg gtatggagct ggaaaaagca accctggaag acctgggtca ggctaaacgt
3481 gttgtgatca acaaagacac caccactatc atcgatggcg tgggtgaaga agctgcaatc
3541 cagggccgtg ttgctcagat ccgtcagcag attgaagaag caacttctga ctacgaccgt
3601 gaaaaactgc aggaacgcgt agcgaaactg gcaggcggcg ttgcagttat caaagtgggt
3661 gctgctaccg aagttgaaat gaaagagaaa aaagcacgcg ttgaagatgc cctgcacgcg
3721 acccgtgctg cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct gatccgcgta
3781 gcgtctaaac tggctgacct gcgtggtcag aacgaagacc agaacgtggg tatcaaagtt
3841 gcactgcgtg caatggaagc tccgctgcgt cagatcgtat tgaactgcgg cgaagaaccg
3901 tctgttgttg ctaacaccgt taaaggcggc gacggcaact acggttacaa cgcagcaacc
3961 gaagaatacg gcaacatgat cgacatgggt atcctggatc caaccaaagt aactcgttct
4021 gctctgcagt acgcagcttc tgtggctggc ctgatgatca ccaccgaatg catggttacc
4081 gacctgccga aaaacgatgc agctgactta ggcgctgctg gcggtatggg cggcatgggt
4141 ggcatgggcg gcatgatgta attgccctgc acctcgcaga aataaacaaa cccccgggca
2.Which organism do these genes come from? How many
sequences of DNA and Protein have been known for
this organism?
Ans:
1.These genes come from Escherichia coli.
2.Escherichia coli K-12 MG1655 complete genome
Accession #: U00096 Length: 4639221 bp G+C content: 50%
4289 protein coding regions and 4405 annotated genes
3.How many BLAST hits you can get if you use the gene
you found for GroEL as the query sequence to search
against non-redundant GenBank + EMBL + DDBJ + PDB
sequence database? Please also show 10 sequences
that produce significant alignments ( you just need
to show their names and ID numbers ).
Distribution of 387 Blast Hits on the Query Sequence
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Escherichia coli K-12 chromosomal region
Escherichia coli K-12 MG1655 section 377
Stenotrophomonas maltophilia GroEL and GroES
Salmonella typhi TY2 heat shock protein GroEL
Klebsiella pneumoniae heat shock protein
Y.enterocolitica gene for heat shock protein 60
Y.enterocolitica hsp60 gene
E.coli groEL (mopA) gene coding for
Y.enterocolitica (0:8) hsp60 gene for HSP60
Sitophilus oryzae principal endosymbiont G.
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