* The side chains of Glu3 and Glu66 are disordered in the crystal structure and were modeled in low-energy conformations.

* A part of the electrostatic stabilization by Arg3 could explained by the absence of unfavorable repulsive forces between E3 and e66 in wild type Bs-CspB.

* E66L stabilization maybe cause by "hydrophobic" effect.