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Techniques
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- | Structure |
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Dynamics |
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- | Energetics |
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IR
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*
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Fingerprint |
*
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H-D exchange |
*
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Ionization states | ||||
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UV/Visible
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*
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Quanltative, DNA conformation |
*
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Follow reaction |
*
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Ligand binding | ||||
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Fluorescence
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**
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Pairwise 2-5 nm (solution) |
***
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Molecular motion ~ps |
**
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Environmental probe, pH | ||||
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NMR
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***
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Pairwise 0.2-1 nm (solution) |
***
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ms - ps |
**
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Ionization states, ligand binding | ||||
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EPR
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*
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Fingerprint around one center |
**
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Diffusion of spin label |
*
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Environmental probe | ||||
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Scattering
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**
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Overall shape |
**
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Net movement of cells and molecules |
*
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Association/dissociation | ||||
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Raman
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*
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Fingerprint |
*
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H-D exchange |
*
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Ionization states | ||||
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Optical Activity
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**
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Secondary structure (solution) |
*
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conformation changes |
*
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Ligand binding | ||||
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Microscopy
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****
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~2nm (solid); ~1mm (solution) |
*
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Photobleaching recovery | ||||||
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Diffraction
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****
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0.2 nm (crystals) |
*
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Temperature factors |
*
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Energy calculation from coordinate |
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