The slow fluoresence unfolding phase is independent of pH and [GdnHCl], and has an activation energy of 20kcal/mol, which strongly suggests that the slow phase correspons to the isomerization of Pro93 and Pro114.
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| Proline Isomerization in Bovine Pancreatic Ribonuclease A. 1. | ||
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Upon addition of GdnHCl,
both mutants exhibit a fast conformational unfolding when monitored by absorbance
and fluorescence, as well as slow phase detected only by fluorescence which
corresponds to the isomerizations of Pro93
and Pro114. The slow fluoresence unfolding phase is independent of pH and [GdnHCl], and has an activation energy of 20kcal/mol, which strongly suggests that the slow phase correspons to the isomerization of Pro93 and Pro114. Next |
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