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Progress Toward Structure Solution by X-ray Crystallography

Explanatory Notes
Summary of Progress, June 9, 2000 Cloned Express Soluble Purified Good
crystals		 Structure
defined		  
Total attempted 117 98 92        
Succeed 116 91 66 47 14 8  
Fail 1 7 26        
Success Rate 99% 93% 72%        
 
Individual Proteins
ProtID aa Org Gene Access# Clone Express Soluble Purified Crystal Structure Related
structure
P001 220 Sce his3 P06633 verified +++ partial purified micro   E
P002 238 Sce rpe1 P46969 verified +++ soluble purified likely   PDB
P003 129 Sce hig1 P40037 verified +++ soluble purified good   PDB,C,A
P004 167 Sce ykl056c P35691 verified +++ soluble purified micro    
P004a 59-167 Sce     fragment +++ soluble        
P005 368 Sce ygr173w P53295 verified +++ partial purified micro   C,E
P006 108 Sce sui1 P32911 verified +++ soluble purified likely   PDB
P006a 20-108 Sce     fragment +++ soluble purified good    
P007 257 Sce ybl036c P38197 verified +++ soluble purified good 1b54 C
P008 228 Sce pdx3 P38075 verified ++ soluble purified good 1cio  
P009 211 Sce yer183c P40099 verified +++ partial purified micro    
P010 307 Sce coq5 P49017 verified +++ soluble purified      
P011 322 Sce snf4 P12904 verified ++ not       A
P012 194 Sce skp1 P52286 verified ++ soluble purified      
P012a 1-??? Sce     fragment ++ soluble purified micro    
P013 407 Sce ynl181w P53878 verified +++ partial        
P014a 124-368 Sce yor221c Q12283 fragment +++ partial        
P015a 885-935 Sce ycr033w Q02397 fragment +++ partial        
P016a 63-182 Sce ypl217c Q08965 fragment +++ partial        
P017 306 Sce ade1 P27616 verified +++ soluble purified likely 1a48  
P018 291 Sce nit3 P49954 verified +++ soluble purified good refining E
P019 177 Sce ybr022w P38218 verified ++ soluble purified micro    
P020 348 Sce hmt1 P38074 verified +++ soluble purified likely    
P021 437 Sce sup45 P12385 verified +++ not       PDB,E
P022 436 Sce abd1 P32783 verified +++ soluble        
P023 483 Sce enp1 P38333 verified ++ not        
P024 322 Sce cdc10 P25342 verified ++ not        
P025 327 Sce nop1 P15646 verified           PDB,B,A,E
P026 391 Sce gpd1 Q00055 verified +++ not        
P027 417 Sce ydl033c Q12093 verified            
P028 266 Sce prp11 Q07350 verified +++ soluble        
P029 275 Sce yla1 P33399 verified ++ soluble purified      
P030 407 Sce qri7 P43122 verified 0          
P031 428 Sce lys20 P48570 verified           A
P032 525 Sce asf2 P32448 verified ++ not        
P033 201 Sce yrb1 P41920 verified ++ soluble purified     PDB
P034 399 Sce fal1 Q12099 verified           PDB,B,A,E
P035 328 Sce hem13 P11353 verified +++ soluble purified micro   B
P036 406 Sce rad55 P38953 verified ? not        
P037 278 Sce rrp1 P35178 verified            
P038 273 Sce bmh2 P34730 verified +++ soluble purified likely   PDB
P039 149 Sce btt1 P40314 verified + not        
P040 274 Sce glo2 Q05584 verified +++ soluble purified     PDB,E
P041 493 Sce arh1 P48360 verified + partial low yld     PDB
P042 493 Sce yel016c P39997 verified 0          
P043 267 Sce ura3 P03962 verified         1dqw  
P044 387 Sce gly1 P37303 verified ++ partial purified      
P045 286 Sce pro3 P32263 verified +++ soluble purified likely    
P046 184 Sce mxr1 P40029 verified ++ soluble purified     B,E
P047 318 Sce prs2 P38620 verified +++ not       PDB,E
P048 142 Sce bur6 P40096 verified + soluble purified good refining PDB
P049 254 Sce sec53 P07283 verified +++ soluble purified micro    
P050 274 Sce rpn12 P32496 verified ++ soluble        
P051 370 Sce pus2 P53167 verified + not       E
P052 482 Sce srm1 P21827 verified ++ soluble       PDB
P053 400 Sce tad1 P53065 verified + not        
P054 361 Sce ufd1 P53044 verified            
P055 287 Sce phb1 P40961 verified ++ not        
P056 246 Sce ski6 P46948 verified +++ not        
P057 309 Sce ser2 P42941 verified            
P058 493 Sce yhl012w P38709 verified ?          
P059 423 Sce yhr033w P38690 verified +++ not        
P060 251 Sce fur1 P18562 verified +++ not       PDB,A,E
P061 129 Sce yhr162w P38857 verified 0          
P062 174 Sce egd2 P38879 verified +++ soluble purified     E
P063 268 Sce rpn10 P38886 verified ++ soluble        
P064 341 Sce yhr210c P38893 verified +++ soluble        
P065 394 Sce yil042c P40530 verified +++ not        
P066 460 Sce dal1 P32375 verified +++ soluble purified micro   E
P067 161 Sce yjl011c P47076 verified +++ not        
P068 299 Sce yjl068c P40363 verified +++ soluble purified good map  
P069 279 Sce asf1 P32447 verified 0         PDB
P070 380 Sce glg2 P47011 verified            
P071 177 Sce bna1 P47096 verified            
P072 453 Sce yjr078w P47125 mutant            
P073 202 Sce met14 Q02196 verified +++ soluble purified micro   PDB
P074 151 Sce ykl002w P36108 verified ++ not        
P075 278 Sce yju2 P28320 verified ++ soluble purified      
P076 382 Sce rad27 P26793 verified           PDB,B,A,E
P077 337 Sce ykl151c P36059 verified           A
P078 466 Sce spe1 P08432 verified +++ soluble       PDB
P079 320 Sce mtd1 Q02046 mutant ++ soluble purified good   PDB
P080 250 Sce ylr022c Q07953 verified +++ soluble purified     E
P081 483 Sce cbf5 P33322 verified ++ not       B,E
P082 328 Sce ylr239c Q06005 verified + partial        
P083 376 Sce ogg1 P53397 verified           PDB
P084 210 Sce rad10 P06838 verified +++ not        
P085 491 Sce hmgs P54839 mutant + not       E
P086 156 Sce ymr002w Q03667 verified + soluble        
P087 249 Sce lys7 P40202 verified +++ soluble purified   1qup  
P088 145 Sce ymr074c Q04773 verified +++ soluble purified      
P089 284 Sce ymr087w Q04299 verified + soluble purified micro    
P090 274 Sce ymr178w Q03219 verified ++ not        
P091 464 Sce ymr293c Q03557 verified ?         E
P092 386 Sce dom34 P33309 verified +++ not       E
P093 456 Sce ynl040w P53960 verified ++ soluble        
P094 226 Sce ynl107w P53930 verified            
P095 240 Sce ynl129w P53915 verified +++ not        
P096 259 Sce ynl168c P53889 verified +++ partial purified good data  
P097 246 Sce ynl200c P40165 verified +++ soluble purified good problem A
P098 433 Sce rpd3 P32561 verified +++ not       PDB
P099 317 Sce ynr027w P53727 verified +++ not        
P100 396 Sce mvd1 P32377 mutant ++ soluble purified good refining  
P101 367 Sce yol010w Q08096 verified           PDB
P102 491 Sce gsh2 Q08220 verified +++ soluble purified good refining PDB
P103 396 Sce spe2 P21182 verified +++ soluble       PDB
P104 420 Sce arg1 P22768 verified +++ soluble        
P105 434 Sce hes1 P35843 verified + soluble        
P106 275 Sce hem4 P06174 verified +++ partial        
P107 150 Sce grx5 Q02784 verified           PDB
P108 456 Sce ypl095c Q02891 indel            
P109 288 Sce idi1 P15496 verified +++ soluble purified      
P110 318 Sce dim1 P41819 verified           PDB,B,E
P111 245 Sce tif6 Q12522 verified ++ soluble purified micro    
P111a 1-224 Sce     fragment ++ soluble purified good refining  
P111-1 228 Mja eif6 Q60357 cloned +++ soluble purified good refining  
P112 405 Sce tif5 P38431             A,E
P113 176 Sce mak3 Q03503              
P114 185 Sce isa2 Q12425             J,C,E
P115 293 Sce spe3 Q12074              
P116 157 Sce nut2 Q06213              
P117 357 Sce hst2 P53686 cloned +++ soluble purified micro    
P118 269 Sce mgm101 P32787              

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