˙Efficiency
Result:
˙construction: pGADGH-A1ΔM9 & pGADGH-A1ΔRG (sequence)
˙β-gal assay
|
|
1
|
2
|
3
|
4
|
5
|
|
A1 Full length
|
3/10
|
5/5
|
5/10
|
10/10
|
1/10
|
|
pGADGH
|
-/10
|
1/4
|
-/10
|
-/10
|
-/10
|
|
ΔM9
|
1/10
|
3/5
|
1/10
|
-/10
|
1/20
|
|
ΔRG
|
-
|
-
|
-
|
-
|
-/10
|
˙Efficiency
|
|
1
|
2
|
3
|
4
|
5
|
|
A1 Full length
|
2322
|
50*
|
474
|
688*
|
50*
|
|
pGADGH
|
4577
|
50*
|
1310
|
3132
|
1850
|
|
ΔM9
|
5292
|
50*
|
1314
|
1944
|
1499
|
|
ΔRG
|
-
|
-
|
-
|
-
|
1148
|
* refers to be contaminated.
