1. Please help him to identify this gene (name, accession #, authors ...... )
Ans: Tetrahymena pyriformis mRNA for hemoglobin
2. Which organism does this gene belong?
Ans: Tetrahymena pyriformis
3. How many nucleotide, protein and structure have been known for this organism?
Ans: Nucleotide (95) , Protein (231) , structure (unknown)
4. Do the Blast search for this gene. List the top 10 most similar sequence.
Ans:
| (1)dbj|D13919.1| | Tetrahymena thermophila mRNA for hemoglobin. |
ttctacaagaaagtcttagcagatgata gagtcaaacattactttaaaaacactaa tatggaacaccaagctaagtaataggaa gattttcttactatgcttttaggaggac ccaatcattataaaggaaaaaacatggc tgaagctcataaaggtatgaacctttaa aactctcactttgacgctatcattgaga atcttgc |
| (2) emb|AL049870.3|CNS0000S | Human chromosome 14 DNA sequHuman chromosome 14 DNA sequence | taatgagagtatttattttatattgttatgta |
| (3)gb|U56081.1|CEU56081 | Caenorhabditis elegans T-BOX 12 (Ce-tbx-12) mRNA | ccatggtacccgatcctcgaattc |
| (4)gb|AF194867.1|AF194867 | Portulaca oleracea NADH dehydrogenase (ndhF) gene | tcatcaaataaatagtaattcta |
| (5)gb|AF194854.1|AF194854 | Portulaca molokiniensis NADH dehydrogenase (ndhF) gene | tcatcaaataaatagtaattcta |
| (6)gb|AF194853.1|AF194853 | Portulaca grandiflora NADH dehydrogenase (ndhF) gene | tcatcaaataaatagtaattcta |
| (7)emb|AJ235828.1|MBR235828 | Mostuea brunonis chloroplast ndhF gene. | tcatcaaataaatagtaattcta |
| (8)gb|AF130178.1|AF130178 | Montinia caryophyllacea NADH dehydrogenase subunit F (ndhF) gene | atcaaataaatagtaattctac |
| (9)gb|AE003647.1|AE003647 | Drosophila melanogaster genomic scaffold 142000013386055 section 40 of 63, complete sequence | atttcaataaaaattattta |
| (10)gb|AE003412.1|DROSADH06 | Drosophila melanogaster, chromosome 2L, region 34C4-36A7 (Adh region), section 6 of 10 of the complete sequence. | atttcaataaaaattattta |
5. Using ORF finder to translate this gene. Show the correct protein sequence.
Ans: translation = "MNKPQTIYEKLGGENAMKAAVPLFYKKVLADERVKHFFKNTDMD HQTKQQTDFLTMLLGGPNHYKGKNMTEAHKGMNLQNLHFDAIIENLAATLKELGVTDA VINEAAKVIEHTRKDMLGK"