Homework 7

Continute from last homework

Help David to analyze the the gene product in ExPasy.

1. Using DNA -> Protein translator to translate gene.

Ans: link here

2. Calaulate pI, molecular weight, amino acid composition of the protein.

Ans: link here

3. Predict α-helice of the protein.

Ans: window size: 9 weight:100%

4. Compute the hydrophobic profile of the protein (show the window and weight you used).

Ans:  window size: 9  weight:100%

Amino acid scale: Hydropathicity.

Author(s): Kyte J., Doolittle R.F.

Reference: J. Mol. Biol. 157:105-132(1982).

Amino acid scale values:

Ala:  1.800  
Arg: -4.500  
Asn: -3.500  
Asp: -3.500  
Cys:  2.500  
Gln: -3.500  
Glu: -3.500  
Gly: -0.400  
His: -3.200  
Ile:  4.500  
Leu:  3.800  
Lys: -3.900  
Met:  1.900  
Phe:  2.800  
Pro: -1.600  
Ser: -0.800  
Thr: -0.700  
Trp: -0.900  
Tyr: -1.300  
Val:  4.200  

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