| 1.
Please help him to identify this gene (name, accession #, authors ......
) |
¡@¡@name:Tetrahymena
pyriformis mRNA for hemoglobin(GI "217409" ¡@¡@¡@¡@[GenBank] )
¡@¡@accession#:D13920
¡@¡ authors:Takagi,T., Iwaasa,H., Yuasa,H., Shikama,K., Takemasa,T. ¡@¡@¡@¡@¡@¡@and
Watanabe,Y.(Biochim. Biophys. Acta 1173 (1), 75-¡@¡@¡@¡@¡@¡@78 (1993)) |
| 2.
Which organism does this gene belong? |
| ¡@¡@
Eukaryota; Alveolata; Ciliophora; Oligohymenophorea; Hymenostomatida;
Tetrahymenina; Tetrahymena. |
| 3.
How many nucleotide, protein and structure have been known for this organism?
|
¡@¡@Nucleotide
(95)
¡@¡@ Protein (231)
¡@¡@ Structure(0) |
| 4.
Do the Blast search for this gene. List the top 10 most similar sequence.
|
|
|
|
NO.1
dbj|D13920.1|TETHEMOGP
Tetrahymena pyriformis mRNA for hemoglobin
Length = 587
Score = 1148 bits (579), Expect = 0.0
Identities = 579/579 (100%) Strand = Plus / Plus
similar sequence
sequence are all the same, so
no need to list.
|
NO.2
dbj|D13919.1|TETHEMOGT
Tetrahymena thermophila mRNA for hemoglobin
Length = 494
Score = 69.9 bits (35), Expect = 1e-09
Identities = 161/203 (79%) Strand = Plus / Plus
similar sequence
Query: 86 ttctacaagaaggtcttagctgatgaaagagtcaagcatttcttcaagaacaccgacatg
145
||||||||||| |||||||| ||||| |||||||| |||| ||| || ||||| | |||
Sbjct: 86 ttctacaagaaagtcttagcagatgatagagtcaaacattactttaaaaacactaatatg
145
Query: 146 gatcaccaaaccaagcaataaactgacttcctcaccatgctcttaggtggtcccaaccat
205
|| |||||| | ||| |||| || || || || ||||| ||||| || ||||| |||
Sbjct: 146 gaacaccaagctaagtaataggaagattttcttactatgcttttaggaggacccaatcat
205
Query: 206 tacaagggtaaaaatatgactgaagctcacaagggtatgaacttgcaaaacttgcacttt
265
|| || || ||||| ||| |||||||||| || ||||||||| | |||||| ||||||
Sbjct: 206 tataaaggaaaaaacatggctgaagctcataaaggtatgaacctttaaaactctcacttt
265
Query: 266 gatgccatcattgaaaaccttgc 288
|| || |||||||| || |||||
Sbjct: 266 gacgctatcattgagaatcttgc 288
¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
|
NO.3
gb|U56081.1|CEU56081
Caenorhabditis elegans T-BOX 12 (Ce-tbx-12) mRNA, complete cds
Length = 1384
Score = 48.1 bits (24), Expect = 0.005
Identities = 24/24 (100%) Strand = Plus / Minus
similar sequence
Query: 564 ccatggtacccgatcctcgaattc 587
||||||||||||||||||||||||
Sbjct: 36 ccatggtacccgatcctcgaattc 13
|
NO.4
emb|AL049870.3|CNS0000S
Human chromosome 14 DNA sequence , complete sequence
Length = 205035
Score = 48.1 bits (24), Expect = 0.005
Identities = 30/32 (93%) Strand = Plus / Plus
similar sequence
Query: 500 taatgagagtatttattgtgtattgttatgta
531
||||||||||||||||| | ||||||||||||
Sbjct: 187601 taatgagagtatttattttatattgttatgta 187632
|
NO.5
gb|AF194867.1|AF194867
Portulaca oleracea NADH dehydrogenase (ndhF) gene, partial cds;
chloroplast gene for chloroplast product
Length = 2087
Score = 46.1 bits (23), Expect = 0.019
Identities = 23/23 (100%) Strand = Plus / Plus
similar sequence
Query: 436 tcatcaaataaatagtaattcta 458
|||||||||||||||||||||||
Sbjct: 122 tcatcaaataaatagtaattcta 144
|
NO.6
gb|AF194854.1|AF194854
Portulaca molokiniensis NADH dehydrogenase (ndhF) gene, partial cds;
chloroplast gene for chloroplast product
Length = 2086
Score = 46.1 bits (23), Expect = 0.019
Identities = 23/23 (100%) Strand = Plus / Plus
similar sequence
Query: 436 tcatcaaataaatagtaattcta 458
|||||||||||||||||||||||
Sbjct: 121 tcatcaaataaatagtaattcta 143
|
NO.7
gb|AF194853.1|AF194853
Portulaca grandiflora NADH dehydrogenase (ndhF) gene, partial cds;
chloroplast gene for chloroplast product
Length = 2141
Score = 46.1 bits (23), Expect = 0.019
Identities = 23/23 (100%) Strand = Plus / Plus
similar sequence
Query: 436 tcatcaaataaatagtaattcta 458
|||||||||||||||||||||||
Sbjct: 185 tcatcaaataaatagtaattcta 207
|
NO.8
emb|AJ235828.1|MBR235828
Mostuea brunonis chloroplast ndhF gene
Length = 2202
Score = 46.1 bits (23), Expect = 0.019
Identities = 23/23 (100%) Strand = Plus / Plus
similar sequence
Query: 436 tcatcaaataaatagtaattcta 458
|||||||||||||||||||||||
Sbjct: 180 tcatcaaataaatagtaattcta 202
|
NO.9
gb|AF130178.1|AF130178
Montinia caryophyllacea NADH dehydrogenase subunit F (ndhF) gene,
partial cds; chloroplast gene for chloroplast product
Length = 2197
Score = 44.1 bits (22), Expect = 0.077
Identities = 22/22 (100%) Strand = Plus / Plus
similar sequence
Query: 438 atcaaataaatagtaattctac 459
||||||||||||||||||||||
Sbjct: 156 atcaaataaatagtaattctac 177
|
NO.10
gb|AE003647.1|AE003647
Drosophila melanogaster genomic scaffold 142000013386055 section 40
of 63, complete sequence
Length = 262205
Score = 40.1 bits (20), Expect = 1.2
Identities = 20/20 (100%) Strand = Plus / Plus
similar sequence
Query: 477 atttcaataaaaattattta 496
||||||||||||||||||||
Sbjct: 42127 atttcaataaaaattattta 42146
|
| 5.
Using ORF finder to translate this gene. Show the correct protein sequence.
|
| FQNKENEQTPNYL*KARRRKCHEGCRPSLLQEGLS**KSQAFLQEHRHGSPNQAIN*LPHHALRWSQPLQ
G*KYD*SSQGYELAKLAL*CHH*KPCCYP*GARCHRCCY*RGC*GHRTHP*GYARQVR*LLLLFIFYYY*
LLLNTSSNK**FYSIKLVRFQ*KLFTVMRVFIVYCYVSFIEDDDQDKSHGTRSSN |
| |
