Homework 6

1. Please help him to identify this gene (name, accession #, authors ...... )

2. Which organism does this gene belong?

3. How many nucleotide, protein and structure have been known for this organism?

4. Do the Blast search for this gene. List the top 10 most similar sequence.

5. Using ORF finder to translate this gene. Show the correct protein sequence.

DEFINITION Tetrahymena thermophila mRNA for hemoglobin.
ACCESSION D13920
VERSION D13920.1 GI:217409.
SOURCE Tetrahymena thermophila cDNA to mRNA.
ORGANISM Tetrahymena thermophila Eukaryota; Alveolata; Ciliophora; Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymena.
AUTHORS Takagi,T., Iwaasa,H., Yuasa,H., Shikama,K., Takemasa,T. and Watanabe,Y.

What we have known about this organism: Nucleotide (95) Protein (231) Structure (0)

Top 10 most similar sequence:
1.dbj|D13920.1|TETHEMOGP Tetrahymena pyriformis mRNA for hemo... 1148 0.0 2.dbj|D13919.1|TETHEMOGT Tetrahymena thermophila mRNA for hem... 70 1e-09 3.emb|AL049870.3|CNS0000S Human chromosome 14 DNA sequence **... 48 0.005 4.gb|U56081.1|CEU56081 Caenorhabditis elegans T-BOX 12 (Ce-tb... 48 0.005 5.gb|AF194867.1|AF194867 Portulaca oleracea NADH dehydrogenas... 46 0.019 6.gb|AF194854.1|AF194854 Portulaca molokiniensis NADH dehydro... 46 0.019 7.gb|AF194853.1|AF194853 Portulaca grandiflora NADH dehydroge... 46 0.019 8.emb|AJ235828.1|MBR235828 Mostuea brunonis chloroplast ndhF ... 46 0.019 9.gb|AF130178.1|AF130178 Montinia caryophyllacea NADH dehydro... 44 0.077 10.gb|AE003647.1|AE003647 Drosophila melanogaster genomic scaf... 40 1.2

Correct protein sequence:
mnkpqtiyek lggenamkaa vplfykkvla dervkhffkn tdmdhqtkqq tdfltmllgg pnhykgknmt eahkgmnlqn lhfdaiienl aatlkelgvt davineaakv iehtrkdmlg