Homework 7
Help David to analyze the the gene product in ExPasy.
1. Using DNA -> Protein translator to translate gene.
2. Calaulate pI, molecular weight, amino acid composition of the protein.
3. Predict a-helice of the protein.
4. Compute the hydrophobic profile of the protein (show the window and weight
you used).
1.K K I K K M N K P Q T I Y E K L G G E N A M K A A V P L F Y K K V L A D E R V K H F F K N T D M D H Q T K Q Stop T D F L T M L L G G P N H Y K G K N M T E A H K G M N L Q N L H F D A I I E N L A A T L K E L G V T D A V I N E A A K V I E H T R K D M L G K Stop D S C C C C L Y S T I I N Y Y L T H H Q I N S N S T Q L N L S D F N K N Y L L Stop _ E Y L L C I V M Y R L L K M M I K T N P M V P D P R I
2. pI: 9.59
Mw: 13555.76
Composition of amino acides:
| Amino acids | Number | Composition |
| Ala (A) | 11 | 9.17 % |
| Arg (R) | 2 | 1.67 % |
| Asn (N) | 9 | 7.50 % |
| Asp (D) | 7 | 5.83 % |
| Cys (C) | 0 | 0.00 % |
| Gln (Q) | 4 | 3.33 % |
| Glu (E) | 8 | 6.67 % |
| Gly (G) | 8 | 6.67 % |
| His (H) | 6 | 5.00 % |
| Ile (I) | 5 | 4.17 % |
| Leu (L) | 12 | 10.00 % |
| Lys (K) | 15 | 12.50 % |
| Met (M) | 7 | 5.83 % |
| Phe (F) | 5 | 4.17 % |
| Pro (P) | 3 | 2.50 % |
| Ser (S) | 0 | 0.00 % |
| Thr (T) | 9 | 7.50 % |
| Trp (W) | 0 | 0.00 % |
| Tyr (Y) | 3 | 2.50 % |
| Val (V) | 6 | 5.00 % |
| Asx (B) | 0 | 0.00 % |
| Glx (Z) | 0 | 0.00 % |
3. My answer
4.Window
size: 9
Relative
weight of the window edges compared to the window center (in %): 100
Profile