Homework 7

Help David to analyze the the gene product in ExPasy.
1. Using DNA -> Protein translator to translate gene.
2. Calaulate pI, molecular weight, amino acid composition of the protein.
3. Predict a-helice of the protein.
4. Compute the hydrophobic profile of the protein (show the window and weight you used).


1.K K I K K M N K P Q T I Y E K L G G E N A M K A A V P L F Y K K V L A D E R V K H F F K N T D M D H Q T K Q Stop T D F L T M L L G G P N H Y K G K N M T E A H K G M N L Q N L H F D A I I E N L A A T L K E L G V T D A V I N E A A K V I E H T R K D M L G K Stop D S C C C C L Y S T I I N Y Y L T H H Q I N S N S T Q L N L S D F N K N Y L L Stop _ E Y L L C I V M Y R L L K M M I K T N P M V P D P R I

2. pI: 9.59
Mw: 13555.76
Composition of amino acides:

Amino acids Number Composition
Ala (A) 11 9.17 %
Arg (R) 2 1.67 %
Asn (N) 9 7.50 %
Asp (D) 7 5.83 %
Cys (C) 0 0.00 %
Gln (Q) 4 3.33 %
Glu (E) 8 6.67 %
Gly (G) 8 6.67 %
His (H) 6 5.00 %
Ile (I) 5 4.17 %
Leu (L) 12 10.00 %
Lys (K) 15 12.50 %
Met (M) 7 5.83 %
Phe (F) 5 4.17 %
Pro (P) 3 2.50 %
Ser (S) 0 0.00 %
Thr (T) 9 7.50 %
Trp (W) 0 0.00 %
Tyr (Y) 3 2.50 %
Val (V) 6 5.00 %
Asx (B) 0 0.00 %
Glx (Z) 0 0.00 %

3. My answer

 

4.Window size: 9
Relative weight of the window edges compared to the window center (in %): 100

Profile