策肈:颠贺侩醚
Title:
Identification of different bacteria by suppressive subtractive hybridization
Abstract:
Development a diagnostic way for the rapid identification of pathogenic bacteria.
Introduction:
Some of the bacterial clones contained sequences common to the majority of "A"strain but different in "B" strain.The difference could be useful for diagnostic test that can identificate pathogenic bacteria.
Method:
Pure genomic DNA from pathogenic bacteria
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cDNA synthesis
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Rsa1 digestion
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Adaptor ligation
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First hybridization
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Second hybridization
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First PCR amplification
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Second PCR amplification
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competent cell ligation
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LB plate incubate
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blue/white screening
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PCR
Result:
The gel electrophoresis picture show the specific sequences amplificated by PCR.
Conclusions:
By using these specific sequences of pathogenic bacteria.It can be the rapid diagnostic test for identification of pathogenic bacteria.