Sequence analysis of MLH1 protein.
- To search protein file in SWISS-PROT Database 
- To use analysis tools in ExPASy
- Deadline - 11/29/2001



1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its (a) accession number, (b) entry name and (c) release date of last modification.
ans:(a)P40692(b)MLH1_HUMAN(c)Release 40, October 2001

2. Give its number of amino acids, molecular weight and theoretical pI.
ans:(a)Number of amino acids:756(b)Molecularweight:84600.98 (c)Theoretical pI: 5.51 

3. Calculate the total number of negatively charged residues and positively charged residues.
ans:Total number of negatively charged residues (Asp + Glu): 104
    Total number of positively charged residues (Arg + Lys): 83

4. Calculate its hydrophobicity (Kyte & Doolittle scale, window size 11, Relative weight 60%).
ans:Ala:  1.800  Arg: -4.500  Asn: -3.500  Asp: -3.500  Cys:  2.500  Gln: -3.500  
Glu: -3.500  Gly: -0.400  His: -3.200  Ile:  4.500  Leu:  3.800  Lys: -3.900  
Met:  1.900  Phe:  2.800  Pro: -1.600  Ser: -0.800  Thr: -0.700  Trp: -0.900  
Tyr: -1.300  Val:  4.200  Asx: -3.500  Glx: -3.500  Xaa: -0.490  

5. Performe the trypsin (higher specificity) cleavage of the protein. (a) How many peptides will you get after cleavage? (b) Give the list of peptides with a mass bigger than 1000 dalton.
ans:(a)33
    (b)mass position #MC peptide sequence 
4615.429 619-659 0 AEMLADYFSLEIDEEGNLIG LPLLIDNYVPPLEGLPIFIL R 
3115.482 576-604 0 LSEPAPLFDLAMLALDSPES GWTEEDGPK 
2862.478 287-311 0 NTHPFLYLSLEISPQNVDVN VHPTK 
2804.404 137-162 0 APPKPCAGNQGTQITVEDLF YNIATR 
2775.324 523-546 0 EMLHNHSFVGCVNPQWALAQ HQTK 
2751.311 689-713 0 QYISEESTLSGQQSEVPGSI PNSWK 
2634.410 500-522 0 IINLTSVLSLQEEINEQGHE VLR 
2625.413 393-416 0 LDAFLQPLSKPLSSQPQAIV TEDK 
2550.328 555-575 0 LSEELFYQILIYDFANFGVL R 
2201.124 733-751 0 HFTEDGNILQLANLPDLYK 
2140.082 342-361 0 MYFTQTLLPGLAGPSGEMVK  
1968.958 426-443 0 QQDEEMLELPAPAEVAAK 
1835.002 101-118 0 GEALASISHVAHVTITTK 
1833.902 85-100 0 LQSFEDLASISTYGFR 
1774.887 312-325 0 HEVHFLHEESILER 
1550.901 19-33 0 IAAGEVIQRPANAIK 
1532.692 362-377 0 STTSLTSSSTSGSSDK 
1525.596 475-487 0 EDSDVEMVEDDSR 
1460.684 242-254 0 MNGYISNANYSVK 
1410.751 605-616 0 EGLAEYIVEFLK 
1408.722 183-195 0 YSVHNAGISFSVK 
1338.730 275-286 0 AIETVYAAYLPK 
1333.627 660-670 0 LATEVNWDEEK 
1327.733 58-69 0 LIQIQDNGTGIR 
1300.686 206-217 0 TLPNASTVDNIR 
1275.703 256-265 0 CIFLLFINHR 
1174.625 714-722 0 WTVEHIVYK 
1165.523 34-43 0 EMIENCLDAK 
1148.550 227-236 0 ELIEIGCEDK 
1119.496 679-687 0 ECAMFYSIR 
1092.517 444-453 0 NQSLEGDTTK 
1091.504 71-79 0 EDLDIVCER 
1003.514 378-385 0 VYAHQMVR