Multiple
Sequence Alignment of MLH1 protein
- To do Multiple Sequence Alignment in Biology Workbench
- To draw phylogenetic tree from alignment
1. Add MLH1_Human protein to the Biology Workbench. Predict its secondary structure
by GOR4.
answer: (link)
2. Do a homology searching of MLH1_Human in Genpept Full Release Database. Import
MLH1-like protein of C. elegans, S. cerevisiae, D. melanogaster, R. norvegicus
and M. musculus to your workbench. Run CLUSTALW to get multiple sequence alignment
for these six proteins.
answer: (link)
3. Perform BOXSHADE program to get a color-coded plot for the results of question
2.
answer: (link)
S43085 DNA mismatch repair protein MLH1 - human
GENPEPT:460627 Saccharomyces cerevisiae DNA mismatch repair (MLH1) gene, complete
GENPEPT:7595954 Mus musculus MutL homolog 1 protein (MLH1) mRNA, complete cds.
GENPEPT:3192877 Drosophila melanogaster mutL homolog (Mlh1) gene, complete cds_
GENPEPT:1724118 Rattus norvegicus mismatch repair protein (MLH1) mRNA, complete
GENPEPT:3880333 C. elegans
4. Draw rooted phylogenetic tree for these proteins.
answer: (link)