1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its (a) accession number, (b) entry name and (b) release date of last modification.

A:(a)Primary accession number P40692

(b)Entry name MLH1_HUMAN


last release date: Release 40, October 2001

you can go here to see the result

2. Give its number of amino acids, molecular weight and theoretical pI.

A: Number of amino acids: 756

Molecular weight: 84600.9

Theoretical pI: 5.51

3. Calculate the total number of negatively charged residues and positively charged residues.

A: Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 83

exercise 2,3 you can go here to see the result

4. Calculate its hydrophobicity (Kyte & Doolittle scale, window size 11, Relative weight 60%).

A: you can go here to see the plot of hydrophobicity

5. Performe the trypsin (higher specificity) cleavage of the protein. (a) How many peptides will you get after cleavage? (b) Give the list of peptides with a mass bigger than 1000 dalton.

A:(a)82;

(b)56

you can go here to see the result of (b)