Multiple Sequence Alignment of MLH1 protein.
- To do Multiple Sequence Alignment in Biology Workbench
- To draw phylogenetic tree from alignment

1. Add MLH1_Human protein to the Biology Workbench. Predict its secondary structure by GOR4.

A: you may go here to see the result


2. Do a homology searching of MLH1_Human in Genpept Full Release Database. Import MLH1-like protein of C. elegans, S. cerevisiae, D. melanogaster, R. norvegicus and M. musculus to your workbench. Run CLUSTALW to get multiple sequence alignment for these six proteins.

A: you may go here to see the result


3. Perform BOXSHADE program to get a color-coded plot for the results of question 2.

A: you may go here to see the result


4. Draw rooted phylogenetic tree for these proteins.

A: you may go here to see the result