Compare MLH1 (answer of assignment 2.6) and mutS (answer of 2.7) sequence.

Ans¡GNo significant similarity was found

Translate the above two gene sequences to protein sequences.

Ans¡Ggi:463988(assignment 2.6)
translation="MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKE
DLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQ
ITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSREL
IEIGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVH
PTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTD
SREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTS
SNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQH
QTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEML
ADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEV
PGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC"
gi|16127994:2855116-2857677(assignment 2.7)
translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDAKRASQLLDISLTKRGASAGEPIPMA
GIPYHAVENYLAKLVNQGESVAICEQIGDPATSKGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYAT
LDISSGRFRLSEPADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFGTRDLVGFG
VENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVTPMGS
RMLKRWLHMPVRDTRVLLERQQTIGALQDFTAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRA
QLETVDSAPVQALREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERLEVRERER
TGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKEYEDKVLTSKGKALALEKQLYEELFDLL
LPHLEALQQSASALAELDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGP
NMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILHNATEYSLVLM
DEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQDGAAS
KSYGLAVAALAGVPKEVIKRARQKLRELESISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWI
YRLKSLV"

Perform protein sequence homology searching for MLH1 in GenBank. Give the 10 highest hits.

Ans¡G
gi|13878583|sp|Q9JK91|MLH1_MOUSE DNA MISMATCH REPAIR PROTEI... 1292 0.0
gi|13591989|ref|NP_112315.1| mismatch repair protein [Rattu... 1289 0.0
gi|4557757|ref|NP_000240.1| mutL homolog 1; mutL (E. coli) ... 1467 0.0
gi|466462|gb|AAA17374.1| (U07418) human homolog of E. coli ... 1466 0.0
gi|604369|gb|AAA85687.1| (U17857) hMLH1 gene product [Homo ... 1453 0.0
gi|12835158|dbj|BAB23172.1| (AK004105) putative [Mus musculus] 753 0.0
gi|13543339|gb|AAH05833.1|AAH05833 (BC005833) Similar to mu... 731 0.0
gi|7304079|gb|AAF59117.1| (AE003838) Mlh1 gene product [Dro... 615 e-175
gi|3192877|gb|AAC19117.1| (AF068257) mutL homolog [Drosophi... 608 e-173
gi|460627|gb|AAA16835.1| (U07187) Mlh1p [Saccharomyces cere... 471 e-132

Compare human MLH1 protein with MLH1 in M. musculus, R. norvegicus and D. melanogaster. Give the pairwise alignment and % of sequence similarity.

Ans¡G
M. musculus
Score = 1292 bits (3344), Expect = 0.0
Identities = 651/760 (85%), Positives = 693/760 (90%), Gaps = 4/760 (0%)

 Query: 1 MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQ 60
          M+FVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKST+IQV+VKEGGLKLIQ
 Sbjct: 1 MAFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTNIQVVVKEGGLKLIQ 60
 Query: 61 IQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTA 120
           IQDNGTGIRKEDLDIVCERFTTSKLQ+FEDLASISTYGFRGEALASISHVAHVTITTKTA
 Sbjct: 61 IQDNGTGIRKEDLDIVCERFTTSKLQTFEDLASISTYGFRGEALASISHVAHVTITTKTA 120
 Query: 121 DGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVV 180
            DGKCAYRASYSDGKL+APPKPCAGNQGT ITVEDLFYNI TRRKALKNPSEEYGKILEVV
 Sbjct: 121 DGKCAYRASYSDGKLQAPPKPCAGNQGTLITVEDLFYNIITRRKALKNPSEEYGKILEVV 180
 Query: 181 GRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAF 240
            GRYS+HN+GISFSVKKQGETV+DVRTLPNA+TVDNIRSIFGNAVSRELIE+GCEDKTLAF
 Sbjct: 181 GRYSIHNSGISFSVKKQGETVSDVRTLPNATTVDNIRSIFGNAVSRELIEVGCEDKTLAF 240
 Query: 241 KMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISP 300
            KMNGYISNANYSVKKCIFLLFINHRLVES +LRKAIETVYAAYLPKNTHPFLYLSLEISP
 Sbjct: 241 KMNGYISNANYSVKKCIFLLFINHRLVESAALRKAIETVYAAYLPKNTHPFLYLSLEISP 300
 Query: 301 QNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMV 360
            QNVDVNVHPTKHEVHFLHEESIL+RVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGE 
 Sbjct: 301 QNVDVNVHPTKHEVHFLHEESILQRVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEAA 360
 Query: 361 KXXXXXXXXXXXXXXDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQ--AIVTEDKTD 418
            +              DKVYA+QMVRTDSR+QKLDAFLQP+S + SQPQ A V +T+
 Sbjct: 361 RPTTGVASSSTSGSGDKVYAYQMVRTDSRDQKLDAFLQPVSSLVPSQPQDPAPVRGARTE 420
 Query: 419 ISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSS--NPRKRHRXX 476
             S RA ++DEEML LPAPAE AA++++LE ++ TS+ ++K PTSS + RKRHR 
 Sbjct: 421 GSPERATREDEEMLALPAPAEAAAESENLERESLMETSDAAQKAAPTSSPGSSRKRHRED 480
 Query: 477 XXXXXXXXXXRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNP 536
                       KEMTAAC PRRRIINLTSVLSLQEEI+E+ HE LRE+L NHSFVGCVNP
 Sbjct: 481 SDVEMVENASGKEMTAACYPRRRIINLTSVLSLQEEISERCHETLREILRNHSFVGCVNP 540
 Query: 537 QWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESG 596
            QWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESG
 Sbjct: 541 QWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESG 600
 Query: 597 WTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIF 656
            WTE+DGPKEGLAEYIVEFLKKKAEMLADYFS+EIDEEGNLIGLPLLID+YVPPLEGLPIF
 Sbjct: 601 WTEDDGPKEGLAEYIVEFLKKKAEMLADYFSVEIDEEGNLIGLPLLIDSYVPPLEGLPIF 660
 Query: 657 ILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTV 716
            ILRLATEVNWDEEKECFESLSKECAMFYSIRKQYI EESTLSGQQS++PGS WKWTV
 Sbjct: 661 ILRLATEVNWDEEKECFESLSKECAMFYSIRKQYILEESTLSGQQSDMPGSTSKPWKWTV 720
 Query: 717 EHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC 756
            EHI+YKA RSH+LPPKHFTEDGN+LQLANLPDLYKVFERC
 Sbjct: 721 EHIIYKAFRSHLLPPKHFTEDGNVLQLANLPDLYKVFERC 760


R. norvegicus
Score = 1289 bits (3336), Expect = 0.0
Identities = 639/758 (84%), Positives = 684/758 (89%), Gaps = 3/758 (0%)

Query: 1 MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQ 60
         MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEM ENCLDAKST+IQVIV+EGGLKLIQ
Sbjct: 1 MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMTENCLDAKSTNIQVIVREGGLKLIQ 60
Query: 61 IQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTA 120
          IQDNGTGIRKEDLDIVCERFTTSKLQ+FEDLA ISTYGFRGEALASISHVAHVTITTKTA
Sbjct: 61 IQDNGTGIRKEDLDIVCERFTTSKLQTFEDLAMISTYGFRGEALASISHVAHVTITTKTA 120
Query: 121 DGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVV 180
           DGKCAYRASYSDGKL+APPKPCAGNQGT ITVEDLFYNI TR+KALKNPSEEYGKILEVV
Sbjct: 121 DGKCAYRASYSDGKLQAPPKPCAGNQGTLITVEDLFYNIITRKKALKNPSEEYGKILEVV 180
Query: 181 GRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAF 240
           GRYS+HN+GISFSVKKQGETV+DVRTLPNA+TVDNIRSIFGNAVSRELIE+GCEDKTLAF
Sbjct: 181 GRYSIHNSGISFSVKKQGETVSDVRTLPNATTVDNIRSIFGNAVSRELIEVGCEDKTLAF 240
Query: 241 KMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISP 300
           KMNGYISNANYSVKKCIFLLFINHRLVES +L+KAIE VYAAYLPKNTHPFLYL LEISP
Sbjct: 241 KMNGYISNANYSVKKCIFLLFINHRLVESAALKKAIEAVYAAYLPKNTHPFLYLILEISP 300
Query: 301 QNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMV 360
           QNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGE V
Sbjct: 301 QNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEAV 360
Query: 361 KXXXXXXXXXXXXXXDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDIS 420
           K              DKV+A+QMVRTDSR+QKLDAF+QP+S+ L SQPQ V ++T+ S
Sbjct: 361 KSTTGIASSSTSGSGDKVHAYQMVRTDSRDQKLDAFMQPVSRRLPSQPQDPVPGNRTEGS 420
Query: 421 SGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSE-MSEKRGPTS-SNPRKRHRXXXX 478
             +A Q+D+E+ ELPAP E AA + SLE ++  G SE ++ +R P+S  + RKRH 
Sbjct: 421 PEKAMQKDQEISELPAPMEAAADSASLERESVIGASEVVAPQRHPSSPGSSRKRHPEDSD 480
Query: 479 XXXXXXXXRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQW 538
                   RKEMTAAC PRRRIINLTSVLSLQEEIN++GHE LREML NH+FVGCVNPQW
Sbjct: 481 VEMMENDSRKEMTAACYPRRRIINLTSVLSLQEEINDRGHETLREMLRNHTFVGCVNPQW 540
Query: 539 ALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWT 598
           ALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRL EPAPLFD AMLALDSPESGWT
Sbjct: 541 ALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLPEPAPLFDFAMLALDSPESGWT 600
Query: 599 EEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFIL 658
           EEDGPKEGLAEYIVEFLKKKA+MLADYFS+EIDEEGNLIGLPLLID+YVPPLEGLPIFIL
Sbjct: 601 EEDGPKEGLAEYIVEFLKKKAKMLADYFSVEIDEEGNLIGLPLLIDSYVPPLEGLPIFIL 660
Query: 659 RLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEH 718
           RLATEVNWDEE ECFESLSKECA+FYSIRKQYI EES LSGQQS++PGS WKWTVEH
Sbjct: 661 RLATEVNWDEE-ECFESLSKECAVFYSIRKQYILEESALSGQQSDMPGSPSKPWKWTVEH 719
Query: 719 IVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC 756
           I+YKA RSH+LPPKHFTEDGN+LQLANLPDL KVFERC
Sbjct: 720 IIYKAFRSHLLPPKHFTEDGNVLQLANLPDLCKVFERC 757

D. melanogaster
Score = 615 bits (1586), Expect = e-175
Identities = 335/751 (44%), Positives = 453/751 (59%), Gaps = 94/751 (12%)

Query: 6 GVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNG 65
         GVIR+LDE VVNRIAAGE+IQRPANA+KE++EN LDA+ST IQV VK GGLKL+QIQDNG
Sbjct: 8 GVIRKLDEVVVNRIAAGEIIQRPANALKELLENSLDAQSTHIQVQVKAGGLKLLQIQDNG 67
Query: 66 TGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCA 125
          TGIR+EDL IVCERFTTSKL  FEDL+ I+T+GFRGEALASISHVAH++I TKTA  KC 
Sbjct: 68 TGIRREDLAIVCERFTTSKLTRFEDLSQIATFGFRGEALASISHVAHLSIQTKTAKEKCG 127
Query: 126 YRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSV 185
           Y+A+Y+DGKL+  PKPCAGNQGT I +EDLFYN+  RR+AL++P+EE+ ++ EV+ RY+V
Sbjct: 128 YKATYADGKLQGQPKPCAGNQGTIICIEDLFYNMPQRRQALRSPAEEFQRLSEVLARYAV 187
Query: 186 HNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGY 245
           HN  + F+++KQG+    +RT   +S  +NIR I+G A+S+EL+E    D+   F+ 
Sbjct: 188 HNPRVGFTLRKQGDAQPALRTPVASSRSENIRIIYGAAISKELLEFSHRDEVYKFEAECL 247
Query: 246 ISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDV 305
           I+  NYS KKC  LLFIN RLVEST+LR +++++YA YLP+  HPF+Y+SL + PQN+DV
Sbjct: 248 ITQVNYSAKKCQMLLFINQRLVESTALRTSVDSIYATYLPRGHHPFVYMSLTLPPQNLDV 307
Query: 306 NVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKXXXX 365
           NVHPTKHEVHFL++E I++ ++Q +E++LLGSN++R ++ Q  LPG   P  +  + 
Sbjct: 308 NVHPTKHEVHFLYQEEIVDSIKQQVEARLLGSNATRTFYKQLRLPG--APDLDETQ---- 361
Query: 366 XXXXXXXXXXDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRAR 425
                      ++Y  +MVRTDS EQKLD FL PL K  S    +   E         A 
Sbjct: 362 -----LADKTQRIYPKEMVRTDSTEQKLDKFLAPLVKSDSGVSSSSSQE---------AS 407
Query: 426 QQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHRXXXXXXXXXXX 485
           +  EE +   A                                  K+ R 
Sbjct: 408 RLPEESFRVTAA---------------------------------KKSREVRLSSVLDMR 434
Query: 486 XRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQT 545
            R E   +   R  + NL  V  + E      HE    M +  SF 
Sbjct: 435 KRVERQCSVQLRSTLKNLVYVGCVDERRALFQHETRLYMCNTRSF--------------- 479
Query: 546 KLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKE 605
                     SEELFYQ +IY+F N   + +S P PL +L +L+L+S  +GWT EDG K 
Sbjct: 480 ----------SEELFYQRMIYEFQNCSEITISPPLPLKELLILSLESEAAGWTPEDGDKA 529
Query: 606 GLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVN 665
            LA+   + L KKA ++ +YF L I E+G L  LP L+  + P +  LP+++LRLATEV+
Sbjct: 530 ELADGAADILLKKAPIMREYFGLRISEDGMLESLPSLLHQHRPCVAHLPVYLLRLATEVD 589
Query: 666 WDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALR 725
           W++E  CFE+  +E A FY+              Q     G+     +WT+EH+++ A +
Sbjct: 590 WEQETRCFETFCRETARFYA--------------QLDWREGATAGFSRWTMEHVLFPAFK 635
Query: 726 SHILPPKHFTEDGNILQLANLPDLYKVFERC 756
            ++LPP    +   I +L NLP LYKVFERC
Sbjct: 636 KYLLPPPRIKD--QIYELTNLPTLYKVFERC 664


Search the conserve domain (CD) for MLH1. Give the position of the CD, name of CD and Pfam ID number.

Ans¡G
Name:DNA_mis_repair, DNA mismatch repair protein. Also known as the mutL/hexB/PMS1 family
Pfam number¡G01119
Position¡G147-327 amino acids


Show multiple alignment of MLH1 conserve domain with 5 sequences from the top of the CD alignment.

Ans¡G

                   10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|
   consensus 1 GTTVEVRDLFYNLPVRRKFLKSPKKEFRKILDLLQRYALIHPNVSFSLTKEG--KALLQL 58 
     query 147 GTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQG--ETVADV 204
    1B63_A 144 GTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNG--KIVRQY 201
gi 8039787 159 GTVVRVEQLFENFPARKRFLGRQSAETTLCRSALIDVSLAHHPVEFRFTVDGthKLTLLS 218
gi 8928214 141 GTIVDVTKIFHNFPARKRFLKQEPIETKMCLKVLEEKIITHPEINFEIN-LN--QKLRKI 197
gi 3914081 141 GTEVEVRDLFFNLPVRRKFLKKEDTERRKVLELIKEYALTNPEVEFTLFSEG--RETLKL 198 
gi 3914082 141 GTEVEVYDLFFNLPARKKFLRKEDTERRKITELVKEYAITNPQVDFHLFSEG--KETLNL 198 
 
                       70        80        90       100       110       120
               ....*....|....*....|....*....|....*....|....*....|....*....|
  consensus 59 KTSP--S-SLKERIRSVFGTAVLKNLIPF--EEKDGDFRIEG-FISSPNVSR-SSRDRQF 111
     query 205 RTLP--NaSTVDNIRSIFGNAVSRELIEIgcEDKTLAFKMNG-YISNANYSV--KKCIFL 259
    1B63_A 202 RAVPegG-QKERRLGAICGTAFLEQALAI--EWQHGDLTLRG-WVADPNHTTpALAEIQY 257
gi 8039787 219 QQTR--K-DRCLETQMLKGDPALFHTIEG--G--DCSFHFHLvLSEPAICRR--ERRGIF 269
gi 8928214 198 YFK---E-SLIDRVQNVYGNVIENNKFRV--LKKEHDNIKIEiFLAPDNFSK-KSKRHIK 250
gi 3914081 199 KKS-----SLKERVEEVFQTKTEELYAER--E--GITLRA---FVSRNQRQG-----KYY 241
gi 3914082 199 KKK-----DLKGRIEEIFESIFEEESSER--E--GIKVRA---FISRNQKRG-----KYY 241
 
                      130       140       150       160       170       180
               ....*....|....*....|....*....|....*....|....*....|....*....|
 consensus 112 LFINGRPVEDKLLLKAIREVYATYLPRGRYPVFVLNLELPPELVDVNVHPDKKEVRLLKE 171
     query 260 LFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHE 319
    1B63_A 258 CYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQS 317
gi 8039787 270 TFVNGRRIFDYGLVQALVLGSEGYFPNGTFPVACLFLTVNSERIDFNIHPAKKEVHLQDY 329
gi 8928214 251 TFVNRRPIDQKDLLEAITNGHSRILSPGNFPICYLFLEINPEYIDFNVHPQKKEVRFYNL 310
gi 3914081 242 VFINKRPIQNKNLKEFLRKVFG------YKTLVVLYAELPPFMVDFNVHPKKKEVNILKE 295
gi 3914082 242 LFVNSRPVYNKNLKEYLKKTFG------YKTIVVLFIDIPPFLVDFNVHPKKKEVKFLKE 295
 
               ....*...
 consensus 172 EEILDLIK 179
     query 320 ESILERVQ 327
    1B63_A 318 RLVHDFIY 325
gi 8039787 330 AHIRHTLS 337
gi 8928214 311 PFLFKLIS 318
gi 3914081 296 RKFLELVR 303
gi 3914082 296 RKIYELIR 303