Untitled Document

HomeWork 9

Comparative Protein Modelling by SWISS-MODEL
- To make a protein model by homology modelling
- Deadline - 1/4/2002

Make a protein structure model of MLH1 based on the protein you find in assignment 7. Show their pairwise alignment.
Length of target sequence: 756 residues
Searching sequences of known 3D structures
	Found 1BKNA.pdb with P(N)=1e-28 Found 1BKNB.pdb with P(N)=8e-27
Sequence identity of templates with target:
	1BKNA.pdb: 46.05 % identity 1BKNB.pdb: 40.9 % identity
Global alignment overview:
	Taget Sequence: \|==================================================\|
	1BKNA.pdb \| ------- 1BKNB.pdb \| -------
Alignment with 1BKNA
Alignment with 1BKNB

How large is the fragment of MLH1 possible to be modeled?
N-terminal 40KD fragment.(Length = 272)

Show three rasmol pictures of your model of MLH1?
Cartoon, color structure.

	1BKNA	1BKNB

Cartoon, color group.

	1BKNA	1BKNB

Spacefill, color structure.

	1BKNA	1BKNB