1. How many hits will you get if you search genes associated with colon
cancer in human genome?
Ans: 17
2. How many loci will you find if you search locus link for human in Genebank?
Ans: 21
3. Give the locus ID and position of MLH1.
Ans: locus ID-- 4292 , position-- 3p21.3
4. Find the %ID of nucleotide sequence for its possible orthologs in mouse.
Ans: 90.5%
5. Find the total number of mutations of MLH1 reported in human gene mutation
database.
Ans: 147
6. Give the DNA sequence of MLH1.
Ans: BASE COUNT 742 a 543 c 601 g 644 t
ORIGIN
1 ggcacttccg ttgagcatct agacgtttcc ttggctcttc tggcgccaaa atgtcgttcg
61 tggcaggggt tattcggcgg ctggacgaga cagtggtgaa ccgcatcgcg gcgggggaag
121 ttatccagcg gccagctaat gctatcaaag agatgattga gaactgttta gatgcaaaat
181 ccacaagtat tcaagtgatt gttaaagagg gaggcctgaa gttgattcag atccaagaca
241 atggcaccgg gatcaggaaa gaagatctgg atattgtatg tgaaaggttc actactagta
301 aactgcagtc ctttgaggat ttagccagta tttctaccta tggctttcga ggtgaggctt
361 tggccagcat aagccatgtg gctcatgtta ctattacaac gaaaacagct gatggaaagt
421 gtgcatacag agcaagttac tcagatggaa aactgaaagc ccctcctaaa ccatgtgctg
481 gcaatcaagg gacccagatc acggtggagg acctttttta caacatagcc acgaggagaa
541 aagctttaaa aaatccaagt gaagaatatg ggaaaatttt ggaagttgtt ggcaggtatt
601 cagtacacaa tgcaggcatt agtttctcag ttaaaaaaca aggagagaca gtagctgatg
661 ttaggacact acccaatgcc tcaaccgtgg acaatattcg ctccatcttt ggaaatgctg
721 ttagtcgaga actgatagaa attggatgtg aggataaaac cctagccttc aaaatgaatg
781 gttacatatc caatgcaaac tactcagtga agaagtgcat cttcttactc ttcatcaacc
841 atcgtctggt agaatcaact tccttgagaa aagccataga aacagtgtat gcagcctatt
901 tgcccaaaaa cacacaccca ttcctgtacc tcagtttaga aatcagtccc cagaatgtgg
961 atgttaatgt gcaccccaca aagcatgaag ttcacttcct gcacgaggag agcatcctgg
1021 agcgggtgca gcagcacatc gagagcaagc tcctgggctc caattcctcc aggatgtact
1081 tcacccagac tttgctacca ggacttgctg gcccctctgg ggagatggtt aaatccacaa
1141 caagtctgac ctcgtcttct acttctggaa gtagtgataa ggtctatgcc caccagatgg
1201 ttcgtacaga ttcccgggaa cagaagcttg atgcatttct gcagcctctg agcaaacccc
1261 tgtccagtca gccccaggcc attgtcacag aggataagac agatatttct agtggcaggg
1321 ctaggcagca agatgaggag atgcttgaac tcccagcccc tgctgaagtg gctgccaaaa
1381 atcagagctt ggagggggat acaacaaagg ggacttcaga aatgtcagag aagagaggac
1441 ctacttccag caaccccaga aagagacatc gggaagattc tgatgtggaa atggtggaag
1501 atgattcccg aaaggaaatg actgcagctt gtaccccccg gagaaggatc attaacctca
1561 ctagtgtttt gagtctccag gaagaaatta atgagcaggg acatgaggtt ctccgggaga
1621 tgttgcataa ccactccttc gtgggctgtg tgaatcctca gtgggccttg gcacagcatc
1681 aaaccaagtt ataccttctc aacaccacca agcttagtga agaactgttc taccagatac
1741 tcatttatga ttttgccaat tttggtgttc tcaggttatc ggagccagca ccgctctttg
1801 accttgccat gcttgcctta gatagtccag agagtggctg gacagaggaa gatggtccca
1861 aagaaggact tgctgaatac attgttgagt ttctgaagaa gaaggctgag atgcttgcag
1921 actatttctc tttggaaatt gatgaggaag ggaacctgat tggattaccc cttctgattg
1981 acaactatgt gccccctttg gagggactgc ctatcttcat tcttcgacta gccactgagg
2041 tgaattggga cgaagaaaag gaatgttttg aaagcctcag taaagaatgc gctatgttct
2101 attccatccg gaagcagtac atatctgagg agtcgaccct ctcaggccag cagagtgaag
2161 tgcctggctc cattccaaac tcctggaagt ggactgtgga acacattgtc tataaagcct
2221 tgcgctcaca cattctgcct cctaaacatt tcacagaaga tggaaatatc ctgcagcttg
2281 ctaacctgcc tgatctatac aaagtctttg agaggtgtta aatatggtta tttatgcact
2341 gtgggatgtg ttcttctttc tctgtattcc gatacaaagt gttgtatcaa agtgtgatat
2401 acaaagtgta ccaacataag tgttggtagc acttaagact tatacttgcc ttctgatagt
2461 attcctttat acacagtgga ttgattataa ataaatagat gtgtcttaac ataaaaaaaa
2521 aaaaaaaaaa
7. Give the DNA sequence of E. coli mismatch repair gene mutS.
1 atgagtgcaa tagaaaattt cgacgcccat acgcccatga tgcagcagta tctcaggctg
61 aaagcccagc atcccgagat cctgctgttt taccggatgg gtgattttta tgaactgttt
121 tatgacgacg caaaacgcgc gtcgcaactg ctggatattt cactgaccaa acgcggtgct
181 tcggcgggag agccgatccc gatggcgggg attccctacc atgcggtgga aaactatctc
241 gccaaactgg tgaatcaggg agagtccgtt gccatctgcg aacaaattgg cgatccggcg
301 accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg ttacgccagg caccatcagc
361 gatgaagccc tgttgcagga gcgtcaggac aacctgctgg cggctatctg gcaggacagc
421 aaaggtttcg gctacgcgac gctggatatc agttccgggc gttttcgcct gagcgaaccg
481 gctgaccgcg aaacgatggc ggcagaactg caacgcacta atcctgcgga actgctgtat
541 gcagaagatt ttgctgaaat gtcgttaatt gaaggccgtc gcggcctgcg ccgtcgcccg
601 ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga atctgcaatt tgggacccgc
661 gatctggtcg gttttggcgt cgagaacgcg ccgcgcggac tttgtgctgc cggttgtctg
721 ttgcagtatg cgaaagatac ccaacgtacg actctgccgc atattcgttc catcaccatg
781 gaacgtgagc aggacagcat cattatggat gccgcgacgc gtcgtaatct ggaaatcacc
841 cagaacctgg cgggtggtgc ggaaaatacg ctggcttctg tgctcgactg caccgtcacg
901 ccgatgggca gccgtatgct gaaacgctgg ctgcatatgc cagtgcgcga tacccgcgtg
961 ttgcttgagc gccagcaaac tattggcgca ttgcaggatt tcaccgccgg gctacagccg
1021 gtactgcgtc aggtcggcga cctggaacgt attctggcac gtctggcttt acgaactgct
1081 cgcccacgcg atctggcccg tatgcgccac gctttccagc aactgccgga gctgcgtgcg
1141 cagttagaaa ctgtcgatag tgcaccggta caggcgctac gtgagaagat gggcgagttt
1201 gccgagctgc gcgatctgct ggagcgagca atcatcgaca caccgccggt gctggtacgc
1261 gacggtggtg ttatcgcatc gggctataac gaagagctgg atgagtggcg cgcgctggct
1321 gacggcgcga ccgattatct ggagcgtctg gaagtccgcg agcgtgaacg taccggcctg
1381 gacacgctga aagttggctt taatgcggtg cacggctact acattcaaat cagccgtggg
1441 caaagccatc tggcacccat caactacatg cgtcgccaga cgctgaaaaa cgccgagcgc
1501 tacatcattc cagagctaaa agagtacgaa gataaagttc tcacctcaaa aggcaaagca
1561 ctggcactgg aaaaacagct ttatgaagag ctgttcgacc tgctgttgcc gcatctggaa
1621 gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg tgctggttaa cctggcggaa
1681 cgggcctata ccctgaacta cacctgcccg accttcattg ataaaccggg cattcgcatt
1741 accgaaggtc gccatccggt agttgaacaa gtactgaatg agccatttat cgccaacccg
1801 ctgaatctgt cgccgcagcg ccgcatgttg atcatcaccg gtccgaacat gggcggtaaa
1861 agtacctata tgcgccagac cgcactgatt gcgctgatgg cctacatcgg cagctatgta
1921 ccggcacaaa aagtcgagat tggacctatc gatcgcatct ttacccgcgt aggcgcggca
1981 gatgacctgg cgtccgggcg ctcaaccttt atggtggaga tgactgaaac cgccaatatt
2041 ttacataacg ccaccgaata cagtctggtg ttaatggatg agatcgggcg tggaacgtcc
2101 acctacgatg gtctgtcgct ggcgtgggcg tgcgcggaaa atctggcgaa taagattaag
2161 gcattgacgt tatttgctac ccactatttc gagctgaccc agttaccgga gaaaatggaa
2221 ggcgtcgcta acgtgcatct cgatgcactg gagcacggcg acaccattgc ctttatgcac
2281 agcgtgcagg atggcgcggc gagcaaaagc tacggcctgg cggttgcagc tctggcaggc
2341 gtgccaaaag aggttattaa gcgcgcacgg caaaagctgc gtgagctgga aagcatttcg
2401 ccgaacgccg ccgctacgca agtggatggt acgcaaatgt ctttgctgtc agtaccagaa
2461 gaaacttcgc ctgcggtcga agctctggaa aatcttgatc cggattcact caccccgcgt
2521 caggcgctgg agtggattta tcgcttgaag agcctggtgt aa