| 1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database.
Give its (a) accession number, (b) entry name and (b) release date of last
modification. |
|
(a)P40692
(b)MLH1_HUMAN Release 40, October 2001
|
| 2. Give its number of amino acids, molecular weight and theoretical pI. |
|
number of amino acidsĄG756 AA
molecular weightĄG84600 Da
theoretical pIĄG5.51
|
| 3. Calculate the total number of negatively charged residues and positively
charged residues. |
|
negatively charged residuesĄGAsp+Glu=104
positively charged residuesĄGArg+Lys=83
|
| 4. Calculate its hydrophobicity (Kyte & Doolittle scale, window size
11, Relative weight 60%). |
| Answer |
| 5. Performe the trypsin (higher specificity) cleavage of the protein.
(a) How many peptides will you get after cleavage? (b) Give the list of
peptides with a mass bigger than 1000 dalton. |
|
(a)33
(b)
4615.429 619-659 0 AEMLADYFSLEIDEEGNLIG LPLLIDNYVPPLEGLPIFIL
R
3115.482 576-604 0 LSEPAPLFDLAMLALDSPES GWTEEDGPK
2862.478 287-311 0 NTHPFLYLSLEISPQNVDVN VHPTK
2804.404 137-162 0 APPKPCAGNQGTQITVEDLF YNIATR
2775.324 523-546 0 EMLHNHSFVGCVNPQWALAQ HQTK
2751.311 689-713 0 QYISEESTLSGQQSEVPGSI PNSWK
2634.410 500-522 0 IINLTSVLSLQEEINEQGHE VLR
2625.413 393-416 0 LDAFLQPLSKPLSSQPQAIV TEDK
2550.328 555-575 0 LSEELFYQILIYDFANFGVL R
2201.124 733-751 0 HFTEDGNILQLANLPDLYK
2140.082 342-361 0 MYFTQTLLPGLAGPSGEMVK
1968.958 426-443 0 QQDEEMLELPAPAEVAAK
1835.002 101-118 0 GEALASISHVAHVTITTK
1833.902 85-100 0 LQSFEDLASISTYGFR
1774.887 312-325 0 HEVHFLHEESILER
1550.901 19-33 0 IAAGEVIQRPANAIK
1532.692 362-377 0 STTSLTSSSTSGSSDK
1525.596 475-487 0 EDSDVEMVEDDSR
1460.684 242-254 0 MNGYISNANYSVK
1410.751 605-616 0 EGLAEYIVEFLK
1408.722 183-195 0 YSVHNAGISFSVK
1338.730 275-286 0 AIETVYAAYLPK
1333.627 660-670 0 LATEVNWDEEK
1327.733 58-69 0 LIQIQDNGTGIR
1300.686 206-217 0 TLPNASTVDNIR
1275.703 256-265 0 CIFLLFINHR
1174.625 714-722 0 WTVEHIVYK
1165.523 34-43 0 EMIENCLDAK
1148.550 227-236 0 ELIEIGCEDK
1119.496 679-687 0 ECAMFYSIR
1092.517 444-453 0 NQSLEGDTTK
1091.504 71-79 0 EDLDIVCER
1003.514 378-385 0 VYAHQMVR
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