| 1. Search human Mlh1 or its homologous proteins in Protein Data Bank (PDB)
database. You just need to search for protein structure, do not find complex
structure. Give its (a) ID number, (b) Name of the molecule, (c) experimental
method for structure determination and (d) Resolution of the structure. |
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(a) ID number¡G1BKN
(b) Name of the molecule¡GMutl
(c) experimental method for structure determination¡GX-ray
Diffraction
(d) Resolution of the structure¡G2.90
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| 2. Show its structure in ribbons form (400x400). |
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| 3. Give the structural informations of this PDB file (a) number of protein
chains, (b) number of helix in one protein chain, (c) the first and last
residues in the helix 6 . |
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(a) number of protein chains¡G2
(b) number of helix in one protein chain¡G11
(c) the first and last residues in the helix 6¡GT
and A
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| 4. Give the Class, Fold, Superfamily and Family of this protein in Structural
Classification of Proteins (SCOP). |
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Class¡GAlpha and beta
proteins (a+b) . Mainly antiparallel beta sheets (segregated alpha and
beta regions)
Fold¡GATPase domain
of HSP90 chaperone/DNA topoisomerase II/histidine kinase. 8-stranded mixed
beta-sheet; 2 layers: alpha/beta
Superfamily¡GATPase
domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
Family¡GDNA gyrase/MutL,
N-terminal domain
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| 5. (a) Show Ramachandran plot for this PDB file. (b) How many residues
are in the most favoured regions of Ramachandran plot? |
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(a) Ramachandran
plot
(b)379
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