Restriction Enzyme Cutting Patterns
 
 

      EcoRI  --- doesn't  cut

      Sau3AI --- 'GATC_   --- 3 Cut(s)   ( 39  ,  162  ,  215 )

      HinfI  --- G'AnT_C  --- 1 Cut(s)   ( 152 )

      MseI   --- T'TA_A   --- 1 Cut(s)   ( 168 )

         



    

                                             Sau3AI 
                                             \                 
    1   aaaaccatcaaatacgttatgctggaatgcaacgaactgatcccgctgttctacgaaacc     60   
        ttttggtagtttatgcaatacgaccttacgttgcttgactagggcgacaagatgctttgg          
            ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
                                                                                
 

   61   tgcccggctggtgaaaacatctgctacgaaatgttcatggttgctaccccgaaagttccg    120   
        acgggccgaccacttttgtagacgatgctttacaagtaccaacgatggggctttcaaggc          
            ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
                                                                                

                                                Sau3AI
                                      HinfI           MseI    
                                      \         \     \   
  121   tgcgaacgtggttgcatcgacgtttgcccggaatcttctctgatcgttaaatacgtttgc    180   
        acgcttgcaccaacgtagctgcaaacgggccttagaagagactagcaatttatgcaaacg          
            ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
                                                                                
                                                            
                                         Sau3AI 
                                         \                                   
  181   tgcaacaccgaccgttgccagtaatccagcgcctgatctctcgaaataaaagccgcatt     240   
        acgttgtggctggcaacggtcattaggtcgcggactagagagctttattttcggcgtaa           
            ^    *    ^    *    ^    *    ^    *    ^    *    ^    *          
                                                                                





Information About Other Restriction Enzyme Sites
Corresponding Polypeptide Sequence
 
 
  
  MLECN ELIPL FYETC PAGEN ICYEM FMVAT PKVPC ERGCI DVCPE SSLIV
  KYVCC NTDRC Q
Gene Identification
 

   There isn't any single gene 100% identical to this DNA sequence, 
   so this DNA is a new toxin.






Please Check the Blast Results
Protein Model
Helices -- Red

Sheets -- Blue

Turns -- Yellow
Red -- Disulfide bonds (3 Bonds)

Yellow -- Cystein (37-48, 9-16, 54-49)
Green -- Basic amino acid (Lys,His,Arg)

Red -- Acidic amino acid (Asp,Glu)




Yin Land
Bioinformatics Main Page
Homework 6