HOMEWORK 6
  • David Liu, same guy from last homework, he also isolated a cDNA clone from snake venom library. The nucleotide sequence of this cDNA clone is shown here:

    AAAACCATCAAATACGTTATGCTGGAATGCAACGAACTGATCCCGCTGTTCTACGAAACCT

    GCCCGGCTGGTGAAAACATCTGCTACGAAATGTTCATGGTTGCTACCCCGAAAGTTCCGTGC

    GAACGTGGTTGCATCGACGTTTGCCCGGAATCTTCTCTGATCGTTAAATACGTTTGCTGCAA

    CACCGACCGTTGCCAGTAATCCAGCGCCTGATCTCTCGAAATAAAAGCCGCATTG


    (1) Please help him to analyze the cutting patterns of following restriction enzyme:
      EcoRI, Sau3AI, HinfI and MseI.


    (2) Please help him to find its corresponding polypeptide sequence.


    (3) Please help him to identify this toxin. Is it a new toxin?


    (4) David would like to see its structure. Could you help him to find structure of this toxin or make a model if it is a new protein? Show structure on your homwpage ( 3 different views).



    Yin Land
    Bioinformatics Main Page