David Liu, a student in department of Life Sciences, got bit by a snake in the backyard of life science building. He was so angry! Thus, he killed the snake and purified several toxins from its venoms. He sequenced one of the toxin, got this sequence:

 1 L K C N K L V P L F Y K T C P A G K N L C Y K M F M V A T P

31 K V P V K R G C I D V C P K S S L L V K Y V C C N T D R C N

(1) Is this a new toxin? Please help him to identify this toxin.

This is not a new protein. This is a CARDIOTOXIN PRECURSOR

(2) Can you find proteins that share sequence homology with this toxin? Show them in multiple alignment form.

(3) Please predict its secondary structure.

(4) Please show its charge distribution.(more information)

1  MKTLLLTTVV VTIVCLDLEY TLKCNKLVPL FYKTCPAGKN LCYKMFMVAT PKVPVKRGCI 
   0+00000000 000000-0-0 00+00+0000 00+00000+0 000+000000 0+000++000 

          61  DVCPKSSLLV KYVCCNTDRC N
          61  -000+00000 +000000-+0 0