Homework 4

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1. Sho-Ming cloned a gene in the lab. The DNA sequence is listed here:

AATTAATTCTCTCAATCAAATCCAATTTTCTCCCTATAAAAACCCTAAGGTCCTATAGTGTTCTATATCC
AACACTAGCTCCTACTCCCTAAAGCATTTATTATATCTTCCCCTAGCTAGATACTTCATTCCACAAATAG
TTTGCAGCTTTTTCTTTTCCTCTAAAACAATGGAAATAGCTGGGAAAATTGCATGCTTTGTGGTATTGTG
CATGGTGGTAGCTGCACCCTGCGCAGAAGCCATAACCTGTGGCCAGGTTACGTCGAATTTGGCACCTT
GTCTTGCTTATCTTAGAAACACGGGGCCTCTGGGACGTTGTTGCGGTGGCGTTAAGGCTCTGGTGAATT
CTGCAAGGACCACAGAAGATCGTCAAATTGCATGCACTTGCCTGAAATCAGCTGCAGGTGCTATTTCTG
GAATCAATTTGGGCAAAGCTGCTGGTCTCCCTAGTACTTGTGGTGTCAATATTCCTTACAAGATCAGCCC
TTCCACTGACTGCTCCAAGTACCTCACTTTTTTTCTCTCTCATGCTATTCTTATCCTTATATTCTATCTGCTT
CATTTTCGCTTATCTTTTAAATTTTTTATTCGGAATCTTTATACCA

Please help him to identify the gene and its protein sequence.

Answer-----

• The gene that Sho-Ming found might be N. tabacum ltp1 gene for lipid transferease.
  

• Retrieve the result from NCBI

• Retrieve the whole report from NCBI

• its protein sequence
  
  MEIAGKIACFVVLCMVVAAPCAEAITCGQVTSNLAPCLAYLRNTGPLGRCCGGVKAL VNSARTTEDRQIACTCLKSAAGAISGINLGKAAGLPSTCGVNIPYKISPSTDCSKVQ
  

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2. Please find the gene that encode the trp repressor. Show its mRNA on your home page.

Answer-----

• mRNA sequence(329---711)

        329         ag cgagtacaac cgggggaggc attttgcttc
        361 ccccgctaac aatggcgaca tattatggcc caacaatcac cctattcagc agcgatggca
        421 gaacagcgtc accaggagtg gttacgtttt gtcgacctgc ttaagaatgc ctaccaaaac
        481 gatctccatt taccgttgtt aaacctgatg ctgacgccag atgagcgcga agcgttgggg
        541 actcgcgtgc gtattgtcga agagctgttg cgcggcgaaa tgagccagcg tgagttaaaa
        601 aatgaactcg gcgcaggcat cgcgacgatt acgcgtggat ctaacagcct gaaagccgcg
        661 cccgtcgagc tgcgccagtg gctggaagag gtgttgctga aaagcgattg a
  

• Retrieve whole file from NCBI
  

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3. How many nucleotide and protein sequence of Lycopersicon esculentum were known?
Please find its class II small heat shock protein mRNA, complete cds.

Answer-------

• 679 nucleotide and 832 protein sequence of Lycopersicon esculentum were known

• its class II small heat shock protein mRNA(81---584), complete cds
  

  
       81                       atgtccaaaa atttctcaat actgaaaatg gatttgaggt
      121 tgttgggtat cgataacaca ccactcttcc acactctcca ccatatgatg gaagctgccg
      181 gtgaagattc cgacaagtct gtcaatgcac catcaaggaa ctatgttcgt gatgctaagg
      241 ccatggctgc tacaccagcg gatgtgaagg agtatcctaa ttcgtatgtt tttgttgtgg
      301 atatgccagg gttgaaatct ggagatatca aagtgcaggt ggaagaagac aatgtgctgt
      361 tgattagtgg tgaaaggaag aggggagaag agaaagaagg tgcaaagttt attaggatgg
      421 agagaagggt tgggaaattc atgaggaagt ttagtctgcc agagaatgcg aatactgatg
      481 caatttctgc agtttgtcaa gatggagttc tgactgttac tgttcagaaa ttgcctcctc
      541 ctgagccaaa gaaacccaaa acaattgagg tgaaagttgc ttga

• Retrieve whole file from NCBI
  

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