Reference 3

J Mol Biol 219: 595-601 (1991)[91278086] 

Crystallization and characterization of the high
molecular weight form of nerve growth factor (7 S
NGF).

N. Q. McDonald & T. L. Blundell

Department of Crystallography, Birkbeck College, London, U.K. 

A high molecular weight form of nerve growth factor (7 S NGF) has been crystallized in
two crystal forms from polyethylene glycol 4000 by the vapour diffusion technique. The
orthorhombic form A belongs to the space group P2(1)2(1)2(1) and has cell dimensions
of a = 95.6, b = 96.5 and c = 147.0 A. With synchrotron X-ray radiation, these crystals
diffract to 2.8 A resolution. They contain an intact 7 S NGF complex in the asymmetric
unit. The tetragonal form B, which grows at similar conditions to the A form, belongs to
the space group P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = 97.4, b =
97.4 and c = 308.3 A. These crystals diffract to 3.6 A resolution and contain one 7 S
complex per asymmetric unit. Native X-ray data have been collected to 3.3 A for the A
form and to 5.0 A for the B form, both using synchrotron radiation. 

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