Homework #7

1) Some functional sites for cardiotoxin:
    a. PS00005 (PDOC00005) Protein kinase C phosphorylation site. 
                    Pattern: [ST]-x-[RK].
                    Matching residues 2-4: K TIK YVMLE
                    Matching residues 34-36: EMFMA TPK VPCER
                    Matching residues 61-63: YVCCN TDR CQZSS
        
    b. PS00272 (PDOC00245) Snake toxins signature. 
                    Pattern:
                    G-C-x(1,3)-C-P-x(8,10)-C-C-x(2)-[PDEN].
                    Matching residues 42-62: VPCER
                    GCIDVCPESSLIVKYVCCNTD RCQZS

2) Enzymatic cleavage of cardiotoxin:
     a. Trpsin cleavage at residues: K/ or R/. (basic residues.)


      1      *       10      *      20       *      30       *       40      *       50
    1 KTIKYVMLECNELIPLFYECPAGENICYEMFMATPKVPCERGCIDVCPES
  51 SLIVKYVCCNTDRCQZSSAZSLEIKA

         As we can see from its amino acid sequence above, the cleavage happens
    at residues: 4, 36, 41, 55, 63, 75, 77.
    *Clickherefor cleaved peptide masses. 

      b. Endoprotease Glu-C Cleavage at residues: E/
         As we can see from its amino acid sequence above, the cleavage happens
    at residues: 9,12,19,24,29,40,49,73,77.
    *Click here for cleaved peptide masses.

 3) Molecular mass = 8665.22 +/- 0.98