Materials  
         
      Chromosome medium (one bottle per student)--- KaryoMAX Peripheral Blood Karyotyping Medium. These bottles usually come in groups of 10 (each bottle containing 5 mL of media).

      Colcemid solution (10 mL bottle) --- KaryoMAX Colcemid liquid

      Giemsa stain (100 mL bottle) --- KaryoMAX Giemsa Stain Stock

      Potassium chloride solution, 0.075M (about 10 mL per student) --- KaryoMAX Potassium
          Chloride Solution, 0.075M
       
       

      Needed for the class:

      Centrifuge

      Glacial acetic acid (ACS grade)

      Absolute methanol (ACS grade)

      Refrigerator

      Incubator (capable of 37oC)

      Frosted glass slides
       
       

      Needed for each person

      Sterile 1 or 5 mL syringe21-guage needle for the syringeGreen-top vacutube21-guage
          multidraw needle (for vactube)15 mL centrifuge tube

      Pasteur pipetteBulb for the pasteur pipette70 percent isopropyl alcohol pad