David Liu, a student in the
department of Lfe Sciences, got the following mRNA sequence from a
rat liver cDNA library.
Unfortunately, he doesn't know how
to use the sequence analysis tools availabled in the internet
since he did not take Bioinformatics before. Could you help him to
do the following analysis?
(1) Find its corresponding
polypeptide
sequence (DNA -> Protein
translation). Answer
(2) Identify this protein. Is
it a new protein? What kind of rat is this protein belong to?
(China, Noway, German.....) Answer
(3) Report the total number of
negatively charged residues and positively charged
residues. Answer
(4) Draw the hydrophobicity
map for this protein using Eisenberg hydrophobicity scale with
window size 7. The relative weight of the window edges compared to
the window center should set to 40%. Answer
(5) Please help him to use
Prosite scanning tool to find out possible
functions or pattern of this protein. Answer
(6) Color the protein by the
hydrophobicity of the amino acids. Answer