Add MLH1_Human protein to the Biology Workbench. Predict its secondary structure by GOR4.
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           >MLH1_HUMAN
Sequence   MSFVAGVIRR LDETVVNRIA AGEVIQRPAN AIKEMIENCL DAKSTSIQVI
Structure  CCCCEEEEEE CCHHHHHHHH HCHHHHHHHH HHHHHHHHHC CCCCCCHHHH

Sequence   VKEGGLKLIQ IQDNGTGIRK EDLDIVCERF TTSKLQSFED LASISTYGFR
Structure  HHHCCCEEEE ECCCCCCCHH HHHHHHHCCC CCCCCCCCHH HHHHCCCCCC

Sequence   GEALASISHV AHVTITTKTA DGKCAYRASY SDGKLKAPPK PCAGNQGTQI
Structure  CCHHHHHHHE EEEEEEECCC CCCCEEEECC CCCCCCCCCC CCCCCCCCEE

Sequence   TVEDLFYNIA TRRKALKNPS EEYGKILEVV GRYSVHNAGI SFSVKKQGET
Structure  EEHHHHHHHH HHHHHHCCCC HHHHHEEEEE ECCCCCCCCE EEEECCCCCE

Sequence   VADVRTLPNA STVDNIRSIF GNAVSRELIE IGCEDKTLAF KMNGYISNAN
Structure  EEEEEECCCC CCCCCEEEEC CCCCCHHHHH HCCCHHHHHH CCCCCEECCC

Sequence   YSVKKCIFLL FINHRLVEST SLRKAIETVY AAYLPKNTHP FLYLSLEISP
Structure  CCCCCEEEEE ECCCCHHHHH HHHHHHHHHH HHCCCCCCCC EEEECCCCCC

Sequence   QNVDVNVHPT KHEVHFLHEE SILERVQQHI ESKLLGSNSS RMYFTQTLLP
Structure  CCCCEEECCC CCHHHHHHHH HHHHHHHHHH HHHHHCCCCC CEEEEEEECC

Sequence   GLAGPSGEMV KSTTSLTSSS TSGSSDKVYA HQMVRTDSRE QKLDAFLQPL
Structure  CCCCCCCCEE EEEEEEEEEC CCCCCCHHHH HHHHHHHHHH HHHHHHHCCC

Sequence   SKPLSSQPQA IVTEDKTDIS SGRARQQDEE MLELPAPAEV AAKNQSLEGD
Structure  CCCCCCCCCE EECCCCCCHH HHHHHHHHHH HHHCCCHHHH HHHHHCCCCC

Sequence   TTKGTSEMSE KRGPTSSNPR KRHREDSDVE MVEDDSRKEM TAACTPRRRI
Structure  CCCCCCHHHC CCCCCCCCCC CCCCCCCCHH HHHHHHHHHH HHHCCCCCEE

Sequence   INLTSVLSLQ EEINEQGHEV LREMLHNHSF VGCVNPQWAL AQHQTKLYLL
Structure  ECCCCHHHHH HHHHHHHHHH HHHHHCCCCE EEEECCCCHH HHHHHHHHHH

Sequence   NTTKLSEELF YQILIYDFAN FGVLRLSEPA PLFDLAMLAL DSPESGWTEE
Structure  HCCCCHHHHH HHHHHHCCCC CCEECCCCCC CHHHHHHHHC CCCCCCCCCC

Sequence   DGPKEGLAEY IVEFLKKKAE MLADYFSLEI DEEGNLIGLP LLIDNYVPPL
Structure  CCCCCCHHHH HHHHHHHHHH HHHHHHHHHH HHCCCCCCCC EEECCCCCCC

Sequence   EGLPIFILRL ATEVNWDEEK ECFESLSKEC AMFYSIRKQY ISEESTLSGQ
Structure  CCCCHHHHHH HHHHCHHHHH HCCCCCCCCC HHHHHCCCCC CCHHHHCCCC

Sequence   QSEVPGSIPN SWKWTVEHIV YKALRSHILP PKHFTEDGNI LQLANLPDLY
Structure  CCCCCCCCCC CCCEEEECHH HHHHHCCCCC CCCCCCCCHH HHHHCCCCCE

Sequence   KVFERC
Structure  EEEEEC

LEGEND:

Alpha Helix = H Beta Sheet = E Random Coil = C

Do a homology searching of MLH1_Human in Genpept Full Release Database. Import MLH1-like protein of C. elegans, S. cerevisiae, D. melanogaster, R. norvegicus and M. musculus to your workbench. Run CLUSTALW to get multiple sequence alignment for these six proteins.
Ans. multiple sequence alignment for these six proteins

Perform BOXSHADE program to get a color-coded plot for the results of question 2.
Ans.

Draw rooted phylogenetic tree for these proteins.
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