Structural Characterizations

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CD

B1 domain

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Rop

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Janus

Tm = 61.9 °C

NMR

1D spectrum of a) B1 domain, b) Rop, and c) Janus

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HSQC spectrum of Janus

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TOCSY spectra of Rop and Janus

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Fluoresence

Fluoresence emission spectra of a) Rop, and b) Janus in the presence and absence of 6M GuHCl