Jya-Wei Cheng
程家維教授

Research Interest: 
My group is actively engaged in the determination of biomolecular structures and functions using multi-dimensional NMR spectroscopy and other biophysical techniques (such as CD, fluorescence, Biosensor, etc.).  During the past years, we have made considerable progress in understanding the the stability, folding, structure, and peptide binding specificity of the SH3 and SH2 domains of Btk, a tyrosine kinase that is involved in X-Linked agammaglobulinemia (XLA) and B cell development.  The success of this work　depended heavily on the protein cloning, expression, purification, and NMR structural study ability of this group.

In addition to the structural and functional studies of Btk, we have determined the solution conformation of the propeptide domain of factor IX and the predicted a-helix region of HIV-1 integrae.  We have also determined the relationship between local helix contents and RNA binding activity of the N-terminal leucine-repeat region of hepatitis delta antigen.  For the DNA/RNA structural studies, we have determined the hydration and solution structures of the DNA/RNA hybrid duplexes, [d(CGC)r(aaa)d(TTTGCG)]₂ and [d(CGC)r(a_ma_ma_m)d(TTTGCG)]₂.  We have also developed a new synthetic method to synthesize ¹⁵N- and ¹³C-labeled nucleosides which can be used in the protein/DNA complex structural studie.

Selected Publications: 

1. WengKY, ChouNJ, and Cheng JW (2008). Triggering vacuum capillaries for pneumatic pumping and metering liquids in point-of-care immunoassays. Lab on a Chip 8, 1216-1219.
2. Cheng HT, Huang KC, Yu HY, Gao KJ, Zhao XX, Li F, Town J, Gordon JR, and Cheng JW (2008). A new protocol for high-yield purification of recombinant human CXCL8((3-72))K11R/G31P expressed in Escherichia coli. Protein Expression and Purification 61, 65-72.
3. Huang KC, Lee MC, Wu CW, Huang KJ, Lei HY, and Cheng JW (2008). Solution structure and neutralizing antibody binding studies of domain III of the dengue-2 virus envelope protein. Proteins-Structure Function and Bioinformatics 70, 1116-1119.
4. Wu JM, Wei SY, Chen HL, Weng KY, Cheng HT, and Cheng, JW (2007). Solution structure of a novel D-ss-naphthylalanine substituted peptide with potential antibacterial and antifungal activities. Biopolymers 88, 738-745.
5. Cheng JW, Wu CW, Lin YT, Her SY, Huang KC, Lee MC, and WuSC (2007). Identification of the neutralizing antibody and heparin binding sites of the domain III of JEV and DENV envelope proteins. Biopolymers 88, 541-541.
6. Cheng JW, Wu JM, Wei SY, Chen HL, Weng KY, Cheng HT, and Choi L (2007). A novel D-beta-naphthylalanine substituted peptide with potential antibacterial and antifungal activities. Biopolymers 88, 590-590.
7. Huang KC, Cheng HT, PaiMT, Tzeng SR, and Cheng JW* (2006). Solution structure and phosphopeptide binding of the SH2 domain from the human Bruton's tyrosine kinase. J. Biomol. NMR. 36, 73-78.
8. Wei SY, Wu JM, Kuo YY, Chen HL, Yip BS, Tzeng SR, and Cheng JW* (2006). Solution structure of a novel tryptophan-rich Peptide with bidirectional antimicrobial activity. J Bacteriol. 188(1), 328-34.
9. Wu CW, Lin YT, Huang KC, and Cheng JW* (2005). 1H, 15N and 13C resonance assignments of the domain III of the Dengue virus envelope protein. J Biomol NMR. 33(1), 76.
10. .Wu KP, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW, Wu SC, and Cheng JW* (2003). Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein. Journal of Biological Chemistry.278, 46007-46013.
11. PaiMT, Yang CS, Tzeng SR, Wang C, and Cheng JW* (2003). 1H, 15N and 13C resonance assignments of the tetratricopeptide repeat (TPR) domain of hSGT. Journal of Biomolecular NMR.26, 381-382.
12. Wu KP, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW, Wu SC, Cheng JW*. Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein Journal of Biological Chemistry 278, 46007-46013 (2003).
13. M.T. Pai, K.C. Huang, S.R. Tzeng, and J.W. Cheng*, 1H, 15N and 13C resonance assignments of the SH2 domain of Bruton's tyrosine kinase Journal of Biomolecular NMR 24, 163-164 (2002).
14. F.T. Lung, J.Y. Tsai, S.Y. Wei, C. Chen, P.P. Roller, and J.W. Cheng, Novel peptide inhibitors for Grb2 SH2 domain and their detection by surface plasmon resonance Journal of Peptide Research 60, 143-149 (2002).
15. M.L. Jain, Y.P. Tsao, N.L. Ho, and J.W. Cheng*, A Facile Synthesis of [N1, NH2-15N2]Adenine, [N3, NH2-15N2]Adenine and [N1, N3, NH2-15N2]Adenine Journal of Organic Chemistry 60, 6472-6475 (2001).
16. Y.P. Tsao, L.Y. Wang, S.T. Hsu, M.L. Jain, S.H. Chou, W.C. Huang, and J.W. Cheng*, The Solution Structure of [d(CGC)r(amamam)d(TTTGCG)]2 Journal of Biomolecular NMR 21, 209-220 (2001).
17. S.R. Tzeng, M.T. Pai, F.T. Lung, C.W. Wu, P.P. Roller, B. Lei, C.J. Wei, S.C. Tu, S.H. Chen, W.J. Soong, and J.W. Cheng*, Stability and Peptide Binding Specificity of BTK SH2 Domain: Molecular Basis for X-Linked Agammaglobulinemia Protein Science 9, 2377-2385 (2000).
18. Y.C. Lou, I.J. Lin, M.T. Pai, and J.W. Cheng*, Solution Structure of an N-Capping Peptide from the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen Archives of Biochemistry and Biophysics 377, 219-227 (2000).
19. S.T. Hsu, M.T. Chou, and J.W. Cheng*, The Solution Structure of [d(CGC)r(aaa)d(TTTGCG)]2: Hybrid Junctions Flanked by DNA Duplexes Nucleic Acids Research 28, 1322-1331 (2000).
20. S.T. Hsu, M.T. Chou, S.H. Chou, W.C. Huang and J.W. Cheng*, Hydration of [d(CGC)r(aaa)d(TTTGCG)]2 Journal of Molecular Biology 295, 1129-1137 (2000).
21. S.R. Tzeng, Y.C. Lou, M.T. Pai, and J.W. Cheng*, Solution Structure of the BTK SH3 Domain Complexed with a Proline-Rich Peptide from p120cbl Journal of Biomolecular NMR 16, 303-312 (2000).
22. Y.C. Lou, F.T. Lung, M.T. Pai, S.R. Tzeng, S.Y. Wei, P.P. Roller, and J.W. Cheng*, Solution Structure and Dynamics of a Nonphosphorelated Cyclic Peptide Inhibitor for the Grb2 SH2 Domain Archives of Biochemistry and Biophysics 372, 309-314 (1999).
23. S.H. Chou, Y.Y. Tseng, Y.R. Chen, and J.W. Cheng, Structural Studies of Symmetric DNA Undecamers Containing Non-Symmetrical Sheared (PuGAPu):(PyGAPy) Motifs Journal of Biomolecular NMR 14, 157-167 (1999).
24. I.J. Lin, Y.C. Lou, M.T. Pai, H.N. Wu and J.W. Cheng*, Solution Structure and RNA Binding Activity of the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen PROTEINS: Structure, Function, and Genetics 37, 121-129 (1999).